Lu Zhizhong, Li Xiling, Li Kai, Yang Siwei, Jiang Fuguo, Li Shuai, Si Haiyan, Li Junmin, Zhao Xiaoguang
（ 454002） Department of Clinical Laboratory, Jiaozuo People's Hospital, Jiaozuo 454002, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2025 Mar 20;56(2):521-527. doi: 10.12182/20250360606.
To analyze the expression levels of miR-27a-3p mRNA and Yes-associated protein 1 () mRNA in breast cancer, and to explore their relationships with clinicopathological features and the survival prognosis of patients.
A total of 130 breast cancer patients who underwent mastectomy in our hospital between January 2019 and January 2021 were enrolled. The expression levels of miR-27a-3p and mRNA in breast tumor tissues and adjacent normal breast tissues were assessed by qRT-PCR. Furthermore, the relationships between their expression and clinicopathological features, as well as the survival prognosis of patients, were investigated.
Compared with adjacent normal breast tissues, the expression of miR-27a-3p mRNA in breast tumor tissues was lower ( < 0.05), while that of mRNA was higher ( < 0.05). A negative correlation was observed between the expression of miR-27a-3p mRNA and mRNA in breast tumor tissues ( = -0.456, < 0.05). The expression of miR-27a-3p mRNA was correlated with tumor diameter, histological grade, tumor staging by the TNM system, lymph node metastasis, and vascular invasion in patients with breast cancer ( < 0.05). The mRNA expression was correlated with histological grade, tumor staging by the TNM system, lymph node metastasis, and vascular invasion ( < 0.05). Kaplan-Meier survival analysis revealed that the 3-year overall survival rate of the miR-27a-3p low-expression group was 71.60% (48/67), which was lower than the 91.50% (54/59) of the miR-27a-3p high-expression group (log-rank = 8.211, = 0.004). The 3-year overall survival rate of the high-expression group was 73.80% (45/61), lower than that of the low-expression group (87.70%, 57/65) (log-rank =4.429, = 0.035). Multivariate regression analysis indicated that lymph node metastasis (hazard ratio [HR] = 1.409; 95% CI, 1.057-1.644; = 0.046), vascular invasion (HR = 1.541; 95% CI, 1.076-1.869; = 0.045), low miR-27a-3p mRNA expression (HR = 0.593; 95% CI, 0.388-0.925; = 0.018), and high mRNA expression (HR = 0.628; 95% CI, 0.405-0.912; = 0.022) were relevant factors affecting the 3-year overall survival of patients with breast cancer.
A significant downregulation of miR-27a-3p mRNA and upregulation of mRNA are observed in breast tumor tissues. The low expression of miR-27a-3p mRNA and the high expression of mRNA are associated with adverse clinicopathological features and poor survival prognosis, and are risk factors affecting the 3-year overall survival of patients with breast cancer. They show promise as new potential therapeutic targets for breast cancer.
分析miR-27a-3p mRNA和Yes相关蛋白1（YAP1）mRNA在乳腺癌中的表达水平，并探讨它们与临床病理特征及患者生存预后的关系。
选取2019年1月至2021年1月在我院接受乳房切除术的130例乳腺癌患者。采用qRT-PCR检测乳腺癌组织及癌旁正常乳腺组织中miR-27a-3p和YAP1 mRNA的表达水平。此外，研究它们的表达与临床病理特征以及患者生存预后的关系。
与癌旁正常乳腺组织相比，乳腺癌组织中miR-27a-3p mRNA的表达较低（P<0.05），而YAP1 mRNA的表达较高（P<0.05）。乳腺癌组织中miR-27a-3p mRNA与YAP1 mRNA的表达呈负相关（r=-0.456，P<0.05）。miR-27a-3p mRNA的表达与乳腺癌患者的肿瘤直径、组织学分级、TNM系统肿瘤分期、淋巴结转移及血管侵犯相关（P<0.05）。YAP1 mRNA表达与组织学分级、TNM系统肿瘤分期、淋巴结转移及血管侵犯相关（P<0.05）。Kaplan-Meier生存分析显示，miR-27a-3p低表达组的3年总生存率为71.60%（48/67），低于miR-27a-3p高表达组的91.50%（54/59）（log-rank χ²=8.211，P=0.004）。YAP1高表达组的3年总生存率为73.80%（45/61），低于YAP1低表达组的87.70%（57/65）（log-rank χ²=4.429，P=0.035）。多因素回归分析表明，淋巴结转移（风险比[HR]=1.409；95%置信区间，1.057-1.644；P=0.046）、血管侵犯（HR=1.541；95%置信区间，1.076-1.869；P=0.045）、miR-27a-3p mRNA低表达（HR=0.593；95%置信区间，0.3 .88-0.925；P=0.018）和YAP1 mRNA高表达（HR=0.628；95%置信区间，0.405-0.912；P=0.022）是影响乳腺癌患者3年总生存的相关因素。
乳腺癌组织中miR-27a-3p mRNA显著下调，YAP1 mRNA上调。miR-27a-3p mRNA低表达和YAP1 mRNA高表达与不良临床病理特征及生存预后不良相关，是影响乳腺癌患者3年总生存的危险因素。它们有望成为乳腺癌新的潜在治疗靶点。
