Najafi M, Khordadmehr M, Baradaran B, Najafi S, Amini M, Asadpour R
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, 51665-1647, Tabriz, Iran.
Immunology Research Center, Tabriz University of Medical Sciences, 51666-14761, Tabriz, Iran.
Arch Razi Inst. 2024 Dec 31;79(6):1249-1256. doi: 10.32592/ARI.2024.79.6.1249. eCollection 2024 Dec.
Breast cancer represents the most frequently diagnosed form of cancer among women on a global scale. In recent years, there has been a notable increase in interest among researchers in exploring alternative therapeutic methods, including stem cell therapy. The objective of this study was to examine the impact of adipose-derived mesenchymal stem cell-conditioned media (AD-MSCs-CM) on apoptosis induction and migration inhibition of breast cancer cells (MDA-MB-231) . In this study, malignant breast cancer cells (MDA-MB-231) and adipose-derived mesenchymal stem cells (AD-MSCs) were cultured separately in DMEM/F12/FBS (15%) culture media under standard conditions. Subsequently, the conditioned media derived from AD-MSCs was introduced to the MDA-MB-231 cells. Following a 24- and 48-hour exposure period, the expression levels of CASP3, KRAS, and MMP9 were evaluated using a quantitative reverse transcription polymerase chain reaction (qRT-PCR) assay. Furthermore, the proliferation and migration abilities of the cancer cells were evaluated using MTT and wound healing assays, respectively. Furthermore, the protein expression of Caspase-3, K-RAS, and MMP-9 was examined using a western blot assay. It is noteworthy that the expression levels of the MMP9 and KRAS genes were significantly reduced following treatment with AD-MSCs-CM in MDA-MB-231 cells. Furthermore, the CASP3 gene expression level was found to have increased significantly in the treated groups. Additionally, the proliferation of MDA-MB-231 cells treated with AD-MSCs-CM was markedly diminished by MTT and wound healing assays. Moreover, the AD-MSCs-CM was observed to induce caspase-3 activation and reduce the protein expression of K-RAS and MMP-9. The results of this study indicate that AD-MSCs-CM may exert an influence on the apoptosis, proliferation, and migration of breast cancer cells. Consequently, it could be proposed as a promising therapeutic strategy for the suppression of breast cancer. However, further testing and research are required to validate these findings and to ascertain the full potential of this approach.
乳腺癌是全球女性中最常被诊断出的癌症形式。近年来，研究人员对探索包括干细胞疗法在内的替代治疗方法的兴趣显著增加。本研究的目的是检测脂肪来源间充质干细胞条件培养基（AD-MSCs-CM）对乳腺癌细胞（MDA-MB-231）凋亡诱导和迁移抑制的影响。在本研究中，恶性乳腺癌细胞（MDA-MB-231）和脂肪来源间充质干细胞（AD-MSCs）在标准条件下分别于DMEM/F12/FBS（15%）培养基中培养。随后，将AD-MSCs来源的条件培养基加入到MDA-MB-231细胞中。在暴露24小时和48小时后，使用定量逆转录聚合酶链反应（qRT-PCR）检测法评估CASP3、KRAS和MMP9的表达水平。此外，分别使用MTT和伤口愈合检测法评估癌细胞的增殖和迁移能力。此外，使用蛋白质免疫印迹检测法检测Caspase-3、K-RAS和MMP-9的蛋白质表达。值得注意的是，MDA-MB-231细胞经AD-MSCs-CM处理后，MMP9和KRAS基因的表达水平显著降低。此外，发现处理组中CASP3基因表达水平显著升高。另外，MTT和伤口愈合检测法显示，AD-MSCs-CM处理的MDA-MB-231细胞的增殖明显减少。此外，观察到AD-MSCs-CM可诱导caspase-3激活并降低K-RAS和MMP-9的蛋白质表达。本研究结果表明，AD-MSCs-CM可能对乳腺癌细胞的凋亡、增殖和迁移产生影响。因此，它可以被认为是一种有前景的抑制乳腺癌的治疗策略。然而，需要进一步的测试和研究来验证这些发现并确定这种方法的全部潜力。
