Kantati Yendubé T, Kodjo Magloire K, Lefranc Benjamin, Eklu-Gadegbeku Kwashie, Leprince Jérôme, Vaudry David
Laboratory of Physiology/Pharmacology, Physiopathology Bioactive Substances and Innocuity Research Unit (PSBI), Faculty of Sciences of Lomé, Lomé 01BP 1515, Togo.
Univ Rouen Normandie, Inserm, Normandie Univ, NorDiC UMR 1239, Rouen F-76000, France.
IBRO Neurosci Rep. 2025 Jun 16;19:110-116. doi: 10.1016/j.ibneur.2025.06.007. eCollection 2025 Dec.
is a medicinal plant of Togolese flora. We have previously reported that leaves dry hydroethanolic extract (SSE) protects cerebellar granule neurons against HO and 6-OHDA-induced cell death and against ethanol neurotoxicity on the cerebellar cortex of 8-day-old Wistar rats. The present study aimed to extend our knowledge of the protective effects of SSE by exploring its anti-apoptotic mechanisms on cultured PC12 cells. Apoptosis was induced by serum depletion before treatment with various concentrations (5-100 µg/mL) of SSE. SSE (20 µg/mL) significantly protected PC12 cells (+51.8 %) against serum depletion-induced PC12 cell death and inhibited Caspase-3/7 (-65.1 %) activity. SSE also significantly increased the expression of the anti-apoptotic factor . Conversely, it repressed the expression of , and . Taken together, these results indicate that inhibition of apoptosis appears to be one of the main mechanisms of neuroprotective effects.
是多哥植物群中的一种药用植物。我们之前曾报道，叶干水乙醇提取物（SSE）可保护小脑颗粒神经元免受HO和6-OHDA诱导的细胞死亡，并抵御乙醇对8日龄Wistar大鼠小脑皮质的神经毒性。本研究旨在通过探索其对培养的PC12细胞的抗凋亡机制，扩展我们对SSE保护作用的认识。在用不同浓度（5-100μg/mL）的SSE处理之前，通过血清剥夺诱导凋亡。SSE（20μg/mL）显著保护PC12细胞（+51.8%）免受血清剥夺诱导的PC12细胞死亡，并抑制Caspase-3/7（-65.1%）活性。SSE还显著增加了抗凋亡因子的表达。相反，它抑制了、和的表达。综上所述，这些结果表明，抑制凋亡似乎是其神经保护作用的主要机制之一。
