Liu Yongqiang, Zhang Lida, Yang Xilang, Xiao Jialei, Ju Hanxun, Li Chengxin, Shu Changlong, Wang Xiangjing, Zhao Junwei, Xiang Wensheng
Key Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, No. 59 Mucai Street, Xiangfang District, Harbin 150030, PR China.
Harbin Academy of Agricultural Sciences, Harbin 150030, PR China.
Int J Syst Evol Microbiol. 2025 Jul;75(7). doi: 10.1099/ijsem.0.006832.
Four actinobacterial strains, designated NEAU-PBA1, NEAU-PB14, NEAU-PB65 and NEAU-PB77, were isolated from the frass of collected from Harbin, Heilongjiang Province, China. These strains were Gram-stain positive, aerobic and produced branched substrate mycelium and aerial hyphae, without producing diffusible pigments in the tested media. Upon maturity, spore chains formed on the aerial hyphae, while the mycelium fragmented with age. The genome sizes of these four strains ranged from 7.41 to 7.49 Mbp, with the genomic DNA G+C content varying from 71.8% to 72.9%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these four strains belonged to the genus and exhibited high sequence similarity with Mg02. The average nucleotide identity and digital DNA-DNA hybridization values between these four isolates exceeded the threshold for species delineation, while the values between the four isolates and their close relatives were below the threshold. The predominant menaquinones in strain NEAU-PBA1 are MK-10 (H, H, H) and MK-10, while the phospholipids included diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphoglycolipid, phosphatidylcholine, one phospholipid and one lipid. The major cellular fatty acids comprised -C, -C, -C and C 9. Based on polyphasic taxonomic analysis, strains NEAU-PBA1, NEAU-PB14, NEAU-PB65 and NEAU-PB77 were identified as a novel species within the genus , and the name sp. nov. was proposed. The type strain is NEAU-PBA1 (=MCCC 1K08877=JCM 37021).
从中国黑龙江省哈尔滨市采集的粪便中分离出四株放线菌，分别命名为NEAU-PBA1、NEAU-PB14、NEAU-PB65和NEAU-PB77。这些菌株革兰氏染色阳性，好氧，产生分枝状基内菌丝和气生菌丝，在测试培养基中不产生可扩散色素。成熟时，气生菌丝上形成孢子链，而菌丝体会随着年龄增长而断裂。这四株菌株的基因组大小在7.41至7.49 Mbp之间，基因组DNA的G+C含量在71.8%至72.9%之间。基于16S rRNA基因序列的系统发育分析表明，这四株菌株属于该属，与Mg02表现出高度的序列相似性。这四株分离株之间的平均核苷酸同一性和数字DNA-DNA杂交值超过了物种划分的阈值，而这四株分离株与其近缘菌株之间的值低于阈值。菌株NEAU-PBA1中的主要甲基萘醌是MK-10（H，H，H）和MK-10，磷脂包括双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、磷酸糖脂、磷脂酰胆碱、一种磷脂和一种脂质。主要的细胞脂肪酸包括-C、-C、-C和C 9。基于多相分类分析，菌株NEAU-PBA1、NEAU-PB14、NEAU-PB65和NEAU-PB77被鉴定为该属内的一个新物种，并提出了新物种的名称。模式菌株是NEAU-PBA1（=MCCC 1K08877=JCM 37021）。
