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新种和新种,从盐湖岸边土壤中分离出的两个新物种。

sp. nov. and sp. nov., two novel species isolated from the shore soil of salt lake.

作者信息

Zheng Yan-Yan, Zhang Xuan, Liu Zi-Xuan, Wang Rui, Phurbu Dorji, Li Ai-Hua

机构信息

Xizang Key Laboratory of Plateau Fungi, Institute of Plateau Biology of Xizang Autonomous Region, Lhasa, Xizang Autonomous Region 850001, PR China.

China General Microbiological Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.

出版信息

Int J Syst Evol Microbiol. 2025 Jun;75(6). doi: 10.1099/ijsem.0.006826.

Abstract

Six Gram-staining-negative, aerobic, non-motile and rod-shaped bacterial strains, designated as D3-2-R+10, C2-6-M1, E1-3-M2, D2-2-M3, D2-2-M2 and D2-2-M1, were isolated from the shore soil of LungmuCo Lake in Xizang Autonomous Region, China. The 16S rRNA gene sequence comparisons confirmed that the six isolates belonged to the genus of the family . The 16S rRNA gene sequence of strains D3-2-R+10, D2-2-M3, D2-2-M2 and D2-2-M1 exhibited the highest similarities to the type strains of LMG 21980(98.1-98.5 %) and NH1 (97.9-98.4 %). Meanwhile, the 16S rRNA gene sequence of strains C2-6-M1 and E1-3-M2 showed the highest similarities to LMG 21980 (98.2-98.7%). The average nt identity and digital DNA-DNA hybridization values among strains D3-2-R+10 and C2-6-M1 and their most closely related species LMG 21980 were all lower than the threshold values for delineating species, indicating that they represent two separate novel species of . The predominant cellular fatty acids of strains D3-2-R+10 and C2-6-M1 included iso-C, summed feature 3 (C 7/C c) and summed feature 9 (iso-C 9/C 10-methyl), and the predominant respiratory quinone was MK-7. The major polar lipids of strain D3-2-R+10 comprised phosphatidylethanolamine, one unidentified phospholipid and five unidentified lipids, while those of strain C2-6-M1 included phosphatidylethanolamine, two unidentified phospholipids and seven unidentified lipids. The genome size of strains D3-2-R+10 and C2-6-M1 was 6.0 and 5.0 Mb, respectively, with the DNA G+C contents of 40.1 and 40.9 mol %. Based on the above descriptions, the six strains were identified as two novel species of the genus , for which the names sp. nov. and sp. nov. were proposed, with the type strains, respectively.

摘要

从中国西藏自治区龙木错湖岸边土壤中分离出6株革兰氏染色阴性、需氧、无运动性的杆状细菌菌株,分别命名为D3-2-R+10、C2-6-M1、E1-3-M2、D2-2-M3、D2-2-M2和D2-2-M1。16S rRNA基因序列比较证实,这6株分离株属于该科的属。菌株D3-2-R+10、D2-2-M3、D2-2-M2和D2-2-M1的16S rRNA基因序列与LMG 21980(98.1-98.5%)和NH1(97.9-98.4%)的模式菌株具有最高的相似性。同时,菌株C2-6-M1和E1-3-M2的16S rRNA基因序列与LMG 21980(98.2-98.7%)具有最高的相似性。菌株D3-2-R+10和C2-6-M1与其最密切相关的物种LMG 21980之间的平均核苷酸同一性和数字DNA-DNA杂交值均低于界定物种的阈值,表明它们代表该属的两个新物种。菌株D3-2-R+10和C2-6-M1的主要细胞脂肪酸包括异-C、总和特征3(C 7/C c)和总和特征9(异-C 9/C 10-甲基),主要呼吸醌为MK-7。菌株D3-2-R+10的主要极性脂类包括磷脂酰乙醇胺、一种未鉴定的磷脂和五种未鉴定的脂类,而菌株C2-6-M1的主要极性脂类包括磷脂酰乙醇胺、两种未鉴定的磷脂和七种未鉴定的脂类。菌株D3-2-R+10和C2-6-M1的基因组大小分别为6.0和5.0 Mb,DNA G+C含量分别为40.1和40.9 mol%。基于上述描述,这6株菌株被鉴定为该属的两个新物种,分别提出了新物种名sp. nov.和sp. nov.,并分别指定了模式菌株。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1bb9/12205151/67230cc0d15a/ijsem-75-06826-g001.jpg

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