Stellwagen Earle, Stellwagen Nancy C
Department of Biochemistry, University of Iowa, Iowa City, Iowa, USA.
Electrophoresis. 2025 Jun;46(11-12):679-686. doi: 10.1002/elps.70005. Epub 2025 Jul 2.
Free solution capillary electrophoresis (CE) has been used to show that non-symmetric, single-stranded DNA oligomers containing 26 nucleotides can exhibit peaks in the electropherograms that correspond to the simultaneous presence of self-dimers and hairpins in the solution. The overlapping hairpin and self-dimer peaks were observed at temperatures close to 15°C in background electrolytes containing at least 80 mM Na ions. With increasing temperature, the self-dimers were converted first into hairpins and then into random coils at still higher temperatures. The results suggest that hairpins can be an intermediary step in the pathway between DNA duplexes and single-strands.
自由溶液毛细管电泳（CE）已被用于表明，含有26个核苷酸的非对称单链DNA寡聚物在电泳图中可呈现出对应于溶液中同时存在自身二聚体和发夹结构的峰。在含有至少80 mM钠离子的背景电解质中，在接近15°C的温度下观察到了重叠的发夹峰和自身二聚体峰。随着温度升高，自身二聚体首先转化为发夹结构，然后在更高温度下转化为无规卷曲。结果表明，发夹结构可能是DNA双链体和单链之间途径中的一个中间步骤。
