Liaquat Marrium, Le Gall Gwenaelle, Scholey Andrew, Pontifex Matthew G, Bastiaanssen Thomaz F S, Muller Michael, Minihane Anne Marie, Vauzour David
Norwich Medical School, University of East Anglia, Norwich, UK.
Centre for Human Psychopharmacology, Swinburne University, Hawthorn, Australia.
Gut Microbes. 2025 Dec;17(1):2526133. doi: 10.1080/19490976.2025.2526133. Epub 2025 Jul 2.
, a key risk factor for Alzheimer's disease, influences gut microbiota and microbial metabolites (e.g. amino acids and dietary fiber (DF) derived short-chain fatty acids (SCFAs)). However, its role in modulating microbiota-driven DF metabolism and its effect on cognitive status remains unclear. This cross-sectional study ( = 170) investigates the association between genotype, DF consumption, and metabolism in individuals with subjective cognitive impairment (SCI) and mild cognitive impairment (MCI) compared to healthy controls (HC). Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) and H NMR metabolomic techniques were used to quantify SCFAs in serum and fecal samples, respectively. Gut microbiota speciation was carried out by 16S rRNA amplicon sequencing. We found that DF intake was significantly associated with genotype and cognitive status, with lower consumption in carriers ( < 0.05) and those with cognitive impairment (SCI and MCI) ( = 0.03). Differences ( < 0.05) in gut microbiota (both α- and β-diversity) and SCFAs were evident between and non- carriers, with stronger associations with DF consumption and cognitive status evident in non- carriers. These findings suggest that targeting DF-induced changes in gut microbiota and serum SCFAs may be an effective strategy for mitigating cognitive impairment, but primarily in non- carriers.
作为阿尔茨海默病的一个关键风险因素，[具体因素未提及]会影响肠道微生物群和微生物代谢产物（例如氨基酸以及膳食纤维（DF）衍生的短链脂肪酸（SCFAs））。然而，其在调节微生物群驱动的DF代谢中的作用及其对认知状态的影响仍不清楚。这项横断面研究（n = 170）调查了主观认知障碍（SCI）和轻度认知障碍（MCI）个体与健康对照（HC）相比，[具体基因未提及]基因型、DF摄入量和代谢之间的关联。分别使用液相色谱串联质谱（LC-MS/MS）和1H NMR代谢组学技术对血清和粪便样本中的SCFAs进行定量。通过16S rRNA扩增子测序进行肠道微生物群物种鉴定。我们发现DF摄入量与[具体基因未提及]基因型和认知状态显著相关，[具体基因未提及]携带者（P < 0.05）以及认知障碍者（SCI和MCI）的摄入量较低（P = 0.03）。[具体基因未提及]携带者和非携带者之间肠道微生物群（α和β多样性）和SCFAs存在差异（P < 0.05），非携带者中与DF摄入量和认知状态的关联更强。这些发现表明，针对DF诱导的肠道微生物群和血清SCFAs变化可能是减轻认知障碍的有效策略，但主要适用于非[具体基因未提及]携带者。
