Jacobs An, Nelissen Inge
Flemish Institute for Technological Research (VITO), Environmental Intelligence, Mol, Belgium.
Methods Mol Biol. 2025;2929:145-152. doi: 10.1007/978-1-0716-4595-6_11.
Multi-array technology allows for multiplex profiling of protein biomarker panels through sandwich immunoassays with high sensitivity, excellent reproducibility, and wide dynamic range. We describe here the detection of secreted cytokine levels in media of an in vitro stimulated human alveolar cell model that vary in a dose- and time-dependent manner. Depending on the analyte concentrations, several sample dilutions can be tested on a single multi-spot plate. Thus, the technology provides an efficient method for broad biomarker profiling.
多阵列技术可通过具有高灵敏度、出色重现性和宽动态范围的夹心免疫测定法对蛋白质生物标志物面板进行多重分析。我们在此描述了在体外刺激的人肺泡细胞模型的培养基中分泌细胞因子水平的检测，这些细胞因子水平呈剂量和时间依赖性变化。根据分析物浓度，可在单个多点板上测试几种样品稀释度。因此，该技术为广泛的生物标志物分析提供了一种有效的方法。
