Gillespie M N, Owasoyo J O, Kiritsy-Roy J A, O'Connor W N, Van Loon G R
Toxicology. 1985 Oct;37(1-2):147-57. doi: 10.1016/0300-483x(85)90120-9.
Excessive cigarette smoking acts synergistically with atheromatous coronary artery disease to greatly enhance the risk of acute myocardial infarction. To explore a possible mechanism of this relation, the present study tested the hypotheses that diet-induced atherosclerosis in rabbits is associated with an increase in myocardial (-)-norepinephrine content and that the increased (-)-norepinephrine can be released by nicotine. Adult male rabbits were rendered atherosclerotic by feeding them a standard laboratory diet enriched with 2% cholesterol. After 12-13 weeks on the diet, hearts were excised and retroperfused according to the Langendorff technique. There were no differences between control and atherosclerotic animals in terms of baseline (-)-norepinephrine concentration in the coronary effluent. However, increases in effluent (-)-norepinephrine concentration provoked by 10 micrograms and 30 micrograms nicotine were significantly greater in atherosclerotic hearts than in controls. Similarly, myocardial tissue from atherosclerotic animals contained significantly more (-)-norepinephrine than controls. These observations suggest that diet-induced atherosclerosis in rabbits is associated with an increase in myocardial (-)-norepinephrine content and that the augmented (-)-norepinephrine pool can be mobilized by nicotine.
过度吸烟与动脉粥样硬化性冠状动脉疾病协同作用，极大地增加了急性心肌梗死的风险。为了探究这种关系的可能机制，本研究检验了以下假设：家兔饮食诱导的动脉粥样硬化与心肌（-）-去甲肾上腺素含量增加有关，且增加的（-）-去甲肾上腺素可被尼古丁释放。成年雄性家兔通过喂食富含2%胆固醇的标准实验室饮食而形成动脉粥样硬化。饮食12 - 13周后，取出心脏并按照Langendorff技术进行逆行灌注。在冠状动脉流出液中，对照动物和动脉粥样硬化动物的基线（-）-去甲肾上腺素浓度没有差异。然而，10微克和30微克尼古丁引起的流出液（-）-去甲肾上腺素浓度增加在动脉粥样硬化心脏中比在对照心脏中显著更大。同样，动脉粥样硬化动物的心肌组织中（-）-去甲肾上腺素含量明显高于对照动物。这些观察结果表明，家兔饮食诱导的动脉粥样硬化与心肌（-）-去甲肾上腺素含量增加有关，且增加的（-）-去甲肾上腺素储备可被尼古丁调动。
