Longnecker D S, Roebuck B D, Curphey T J, Lhoste E, Coon C I, MacMillan D
Environ Health Perspect. 1986 Sep;68:197-201. doi: 10.1289/ehp.8668197.
The response of LEW and F344 strain rats to the pancreatic carcinogen azaserine was compared using the size and number of azaserine-induced acidophilic acinar cell foci and nodules as parameters in a 4-month experiment. A second experiment compared the effect of corn oil intake by gavage and dietary routes on the growth of azaserine-induced pancreatic lesions in LEW rats. A third experiment tested the activity of benzyl acetate in regard to its ability to induce acinar cell foci or to promote the growth of such foci in azaserine-treated rats. The results showed that equivalent doses of azaserine induce two to seven times more foci in LEW than in F344 rats, and that LEW rats have a higher incidence of "spontaneous" foci than F344 rats. Azaserine-treated LEW rats that were given 5 mL corn oil/kg body weight 5 days per week by gavage developed more acinar cell foci than rats fed a basal diet (chow). Addition of an equivalent amount of corn oil to chow had a similar effect of enhancing the development of foci. Rats of neither strain developed acinar cell foci when benzyl acetate was given by gavage or in the diet nor was there evidence that benzyl acetate has a significant effect on the development of foci in azaserine-treated rats. These studies also demonstrate that the azaserine/rat model of pancreatic carcinogenesis which was developed in LEW rats can be adapted for use with F344 rats.
在一项为期4个月的实验中，以偶氮丝氨酸诱导的嗜酸性腺泡细胞灶和结节的大小及数量为参数，比较了LEW和F344品系大鼠对胰腺致癌物偶氮丝氨酸的反应。第二项实验比较了通过灌胃和饮食途径摄入玉米油对LEW大鼠偶氮丝氨酸诱导的胰腺病变生长的影响。第三项实验测试了乙酸苄酯在偶氮丝氨酸处理的大鼠中诱导腺泡细胞灶或促进此类病灶生长的能力。结果表明，等量的偶氮丝氨酸在LEW大鼠中诱导的病灶数量是F344大鼠的两到七倍，并且LEW大鼠“自发”病灶的发生率高于F344大鼠。每周5天通过灌胃给予5 mL玉米油/千克体重的偶氮丝氨酸处理的LEW大鼠，比喂食基础饮食（普通饲料）的大鼠产生更多的腺泡细胞灶。在普通饲料中添加等量的玉米油也有类似的促进病灶发展的效果。当通过灌胃或饮食给予乙酸苄酯时，两个品系的大鼠均未形成腺泡细胞灶，也没有证据表明乙酸苄酯对偶氮丝氨酸处理的大鼠病灶发展有显著影响。这些研究还表明，在LEW大鼠中建立的偶氮丝氨酸/大鼠胰腺癌发生模型可适用于F344大鼠。
