Hart B A, Cherian M G, Angel A
Toxicology. 1985 Oct;37(1-2):171-9. doi: 10.1016/0300-483x(85)90122-2.
Cadmium and metallothionein (MT) levels were measured in lungs derived from Lewis rats exposed 0, 5, 10, 15, 20, 24, or 30 times to a Cd acetate aerosol (1.6 mg/m3). Cd burden rose from 2.21 micrograms in control lungs to 42.55 micrograms in lungs of animals exposed 30 times to the Cd aerosol. Pulmonary MT, expressed as nmoles Cd-thionein, paralleled Cd retention. Levels increased from 7.5 nmol in control animals to a value of 340.5 nmol in animals exposed 30 times to Cd. Energy dispersive X-ray microanalysis and the laser micro-probe analyzer were used to localize Cd within the lung. Lung tissue was prepared by a variety of chemical and physical fixation procedures. Cd localization proved unsuccessful by these techniques, presumably because the cellular Cd levels were below the limits of detectability. Using a immunohistochemical procedure which employed an antibody to MT, pulmonary MT was found in alveolar epithelial cells, fibroblasts, and lymphocytes. The MT was restricted to the nuclei of these cells.
对暴露于醋酸镉气雾剂（1.6毫克/立方米）0、5、10、15、20、24或30次的Lewis大鼠的肺组织进行了镉和金属硫蛋白（MT）水平的测量。镉负荷从对照肺组织中的2.21微克增加到暴露于镉气雾剂30次的动物肺组织中的42.55微克。以纳摩尔镉-硫蛋白表示的肺MT与镉潴留情况平行。水平从对照动物中的7.5纳摩尔增加到暴露于镉30次的动物中的340.5纳摩尔。使用能量色散X射线微分析和激光微探针分析仪在肺内定位镉。通过各种化学和物理固定程序制备肺组织。通过这些技术未能成功定位镉，推测是因为细胞内镉水平低于可检测限度。使用一种采用抗MT抗体的免疫组织化学程序，在肺泡上皮细胞、成纤维细胞和淋巴细胞中发现了肺MT。MT局限于这些细胞的细胞核中。
