Takizawa Y, Matsuda Y, Yamasita J
Toxicol Lett. 1985 Sep;27(1-3):27-34. doi: 10.1016/0378-4274(85)90116-x.
Recent studies have indicated that administration of [14C]butylated hydroxyanisole (BHA) to rats, either orally or by intraperitoneal (i.p.) injection, resulted in high retention of radioactivity in the forestomach. The present study was undertaken to investigate the fate of [14C]BHA in non-rodents. 2 Groups of 5-mth-old male beagle dogs were fed a diet containing either 3% or 0.03% BHA for 7 days, and were injected i.p. with 3-tert-[methyl-14C]butyl-4-hydroxyanisole (Amersham International) at a dose of 30 muCi/kg. On the 7th day after [14C]BHA injection, all dogs were killed after fasting overnight, and the liver, kidney, heart, fat tissue and stomach were collected for radioanalysis. An additional 3 beagles served as control group. The fate of BHA after the single i.p. injection of [14C]BHA was examined by the determination of 14C-radioactivity in whole body, blood, urine, feces and several tissues. Blood, urine and feces samples were collected daily for 7 days. Blood samples were collected at intervals for 24 h. BHA was rapidly taken up in the bloodstream, and 50-80% of the total radioactivity was recovered in the urine within 2 days. 15-30% Appeared in the feces within 2 days. The tissue distribution of radioactivity 7 days after [14C]BHA injection showed only a small portion remaining in the stomach (0.16-0.19% of dose/g), liver (0.3-1.7%) and other tissues (0.02%). The radio-activity was almost evenly distributed in the three parts of the stomach (cardia, corpus and pylorus). These findings are in contrast with the previous data in rats that BHA can accumulate in high concentrations in the forestomach.
最近的研究表明，给大鼠口服或腹腔注射[14C]丁基羟基茴香醚（BHA）后，前胃中放射性的保留率很高。本研究旨在调查[14C]BHA在非啮齿动物体内的去向。将两组5个月大的雄性比格犬分别喂食含3%或0.03%BHA的饲料7天，并以30μCi/kg的剂量腹腔注射3-叔丁基-[甲基-14C]丁基-4-羟基茴香醚（阿默沙姆国际公司）。在注射[14C]BHA后的第7天，所有犬只在禁食过夜后处死，收集肝脏、肾脏、心脏、脂肪组织和胃进行放射性分析。另外3只比格犬作为对照组。通过测定全身、血液、尿液、粪便和几种组织中的14C放射性，研究了单次腹腔注射[14C]BHA后BHA的去向。连续7天每天收集血液、尿液和粪便样本。每隔一段时间收集血液样本，持续24小时。BHA迅速被吸收进入血液，两天内尿液中回收了总放射性的50-80%。两天内15-30%出现在粪便中。注射[14C]BHA7天后放射性的组织分布显示，只有一小部分留在胃（剂量/克的0.16-0.19%）、肝脏（0.3-1.7%）和其他组织（0.02%）中。放射性几乎均匀分布在胃的三个部分（贲门、胃体和幽门）。这些发现与之前在大鼠中的数据形成对比，在大鼠中BHA可在前胃中高浓度积累。
