Grisolia-Romero Mariela, Faya Marcela, Marchetti Cynthia, Belotti Eduardo, Priotto Marcelo, Rosu Silvana, Gobello Cristina
Instituto de Investigaciones en Recursos Naturales y Sustentabilidad José Sanchez Labrador S. J, Universidad Católica de Córdoba, Argentina; CONICET, Argentina.
Laboratorio de Biología Celular y Molecular Aplicada, ICiVet-Litoral, Universidad Nacional del Litoral, Argentina; CONICET, Argentina.
Theriogenology. 2025 Oct 15;246:117562. doi: 10.1016/j.theriogenology.2025.117562. Epub 2025 Jun 28.
A thorough understanding of the effect of exogenous light could offer a natural method for reproduction manipulation in the domestic cat. The aim of this study was to describe and compare the effect of a long (LP) and a short (SP) artificial photoperiod on testicular, hormonal and seminal parameters. Male cats were housed in windowless rooms and exposed to a SP (6:18 L/D; n = 4) or a LP (16:8 L/D; n = 4) for three months. Blood and semen samples were collected at the end of the third month before castrations. Testes were gross, histologically and immunohistochemically evaluated. Serum melatonin was higher in the SP than in LP (989.03 ± 70.58 vs. 800.91 ± 93.80 pg/ml, respectively; P < 0.05). Neither anti-mullerian hormone (AMH; 5.81 ± 1.80 vs. 6.14 ± 0.78 ng/ml, respectively; P > 0.05) nor testosterone (5.41 ± 2.45 vs. 3.74 ± 2.11 ng/ml, respectively; P > 0.1) differed between SP and LP. There was no correlation between melatonin and other hormones while testosterone and AMH were negatively correlated (r = - 0.94; P < 0.01). Sperm abnormalities were lower in LP than in the SP (P < 0.05). Seminiferous tubular diameter (P < 0.1) and tubular epithelium height (P < 0.05) were larger in LP than in SP. Elongated spermatids (P < 0.05), spermatozoa (P < 0.01) as well as the meiotic index (P < 0.05) and Leydig cells (P < 0.01) increased in LP. There was a tendency to a higher percentage of PCNA-positive germ cells in the LP (P < 0.1). Caspase-3 positive percentage was higher in SP in both the nucleus (P < 0.05) and cytoplasm (P < 0.05). No differences in vimentin immunoreactivity were found between groups.
深入了解外源光的作用可为家猫繁殖调控提供一种自然方法。本研究旨在描述和比较长光照周期（LP）和短光照周期（SP）对睾丸、激素和精液参数的影响。雄性猫被饲养在无窗房间中，分别接受SP（6:18光/暗；n = 4）或LP（16:8光/暗；n = 4）处理三个月。在去势前第三个月末采集血液和精液样本。对睾丸进行大体、组织学和免疫组织化学评估。SP组血清褪黑素水平高于LP组（分别为989.03±70.58与800.91±93.80 pg/ml；P < 0.05）。抗苗勒管激素（AMH；分别为5.81±1.80与6.14±0.78 ng/ml；P > 0.05）和睾酮（分别为5.41±2.45与3.74±2.11 ng/ml；P > 0.1）在SP组和LP组之间无差异。褪黑素与其他激素之间无相关性，而睾酮与AMH呈负相关（r = - 0.94；P < 0.01）。LP组精子异常率低于SP组（P < 0.05）。LP组生精小管直径（P < 0.1）和小管上皮高度（P < 0.05）大于SP组。LP组延长型精子细胞（P < 0.05）、精子（P < 0.01）以及减数分裂指数（P < 0.05）和睾丸间质细胞（P < 0.01）增加。LP组增殖细胞核抗原（PCNA）阳性生殖细胞百分比有升高趋势（P < 0.1）。SP组细胞核（P < 0.05）和细胞质（P < 0.05）中半胱天冬酶-3阳性百分比均较高。各组波形蛋白免疫反应性无差异。
