Yu Tan, Songyan Li, Honglin Liu, Di Huang, Yiqun Tian, Rui Qu, Hailin Tian, Shichao Zhang, Yuanfei Lv, Yingping Huang, Liu Huigang
College of Basic Medical Science, China Three Gorges University, Yichang, 443002, China.
Engineering Research Center of Eco-environmental in Three Gorges Reservoir Region, Ministry of Education, China Three Gorges University, Yichang, 443002, China.
Chemosphere. 2025 Sep;385:144548. doi: 10.1016/j.chemosphere.2025.144548. Epub 2025 Jul 1.
Glyphosate(GLY), as the most widely used herbicide globally, has attracted much attention for its potential toxicity, especially reproductive toxicity. Its metabolite aminomethylphosphonic acid (AMPA) also has biological activity and may remain in the body for a long time with stronger toxic effects. In this study, zebrafish (Danio rerio) were exposed to GLY or AMPA at the concentration of 0, 0.7, 7 and 35 mg/L for 21 d. Egg production, fertilization rate and hatching rate were decreased in GLY and AMPA exposure at the concentration of 7 and 35 mg/L (p < 0.05). Egg production was more significantly decreased in AMPA than GLY exposure at the concentration of 7 mg/L (p < 0.01). The number of mature eggs and sperm decreased in GLY AMPA groups and mature eggs in AMPA treatment was less than GLY (0.7 and 7 mg/L). Sex steroid hormones levels (E and T) and VTG reduced in both GLY and AMPA exposure. Furthermore, expression of the ar, erα, star, cyp19a1a and hsd17b3 suggesting that GLY and AMPA exhibited an estrogenic effect and AMPA showed stronger influence. Our study multi-dimensionally (morphology and molecular biology) explored the differences in reproductive toxicity between glyphosate and AMPA in zebrafish. In particular, AMPA showed more obvious toxicity than GLY, providing a comprehensive understanding of the differences in reproductive toxicity risks between GLY and AMPA.
草甘膦（GLY）作为全球使用最广泛的除草剂，因其潜在毒性，尤其是生殖毒性而备受关注。其代谢产物氨甲基膦酸（AMPA）也具有生物活性，且可能在体内长时间留存并具有更强的毒性作用。在本研究中，将斑马鱼（Danio rerio）暴露于浓度为0、0.7、7和35mg/L的GLY或AMPA中21天。在7和35mg/L浓度下暴露于GLY和AMPA时，产卵量、受精率和孵化率均下降（p<0.05）。在7mg/L浓度下，AMPA暴露组的产卵量比GLY暴露组下降更显著（p<0.01）。GLY和AMPA组的成熟卵和精子数量减少，且在AMPA处理组中成熟卵数量少于GLY组（0.7和7mg/L）。GLY和AMPA暴露均导致性类固醇激素水平（E和T）及卵黄蛋白原降低。此外，ar、erα、star、cyp19a1a和hsd17b3的表达表明GLY和AMPA具有雌激素效应，且AMPA的影响更强。我们的研究从多维度（形态学和分子生物学）探讨了草甘膦和AMPA对斑马鱼生殖毒性的差异。特别是，AMPA的毒性比GLY更明显，为全面了解GLY和AMPA生殖毒性风险差异提供了依据。
