Tehrani Ghazaleh Mazaheri, Sofroniou Constantina, Singh Malika, Mahmoudi Najet, Huband Steven, Davies Owen, Martin-Fabiani Ignacio
Department of Materials, Loughborough University, LE11 3TU Loughborough, United Kingdom.
School of Sport, Exercise, and Health Sciences, Loughborough University, LE11 3TU Loughborough, United Kingdom.
J Colloid Interface Sci. 2025 Dec;699(Pt 2):138267. doi: 10.1016/j.jcis.2025.138267. Epub 2025 Jun 23.
In this study, we advance the understanding of the interactions between liposomes and gold nanoparticles (AuNPs), aiming to provide a scientific foundation for their potential application in nanomedicine, diagnostics, biosensing, and others. We tune the composition of the liposomes, using blends of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) or 2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) lipids with anionic (1,2-dioleoyl-sn-glycero-3-phospho-l-serine, DOPS) or cationic (1,2-dioleoyl-3-trimethylammonium-propane, DOTAP) lipids, to investigate the effect of surface charge and membrane rigidity on their interactions with AuNPs. Via a combination of spectroscopy, microscopy and scattering techniques, we shed light on the state of AuNPs aggregation in these systems as well as the nature and strength of liposome-AuNP and AuNP-AuNP interactions. Notably, through small-angle neutron scattering (SANS) experiments we demonstrate alterations to the lipid membranes induced by AuNPs, leading to liposome shrinkage and the formation of multilamellar liposomes in two of the systems.
在本研究中，我们深化了对脂质体与金纳米颗粒（AuNPs）之间相互作用的理解，旨在为它们在纳米医学、诊断学、生物传感及其他领域的潜在应用提供科学依据。我们通过使用1,2 - 二棕榈酰 - sn - 甘油 - 3 - 磷酸胆碱（DPPC）或二油酰 - sn - 甘油 - 3 - 磷酸胆碱（DOPC）脂质与阴离子脂质（1,2 - 二油酰 - sn - 甘油 - 3 - 磷酸 - L - 丝氨酸，DOPS）或阳离子脂质（1,2 - 二油酰 - 3 - 三甲基铵丙烷，DOTAP）的混合物来调整脂质体的组成，以研究表面电荷和膜刚性对其与AuNPs相互作用的影响。通过光谱学、显微镜和散射技术的结合，我们揭示了这些系统中AuNPs的聚集状态以及脂质体 - AuNP和AuNP - AuNP相互作用的性质和强度。值得注意的是，通过小角中子散射（SANS）实验，我们证明了AuNPs对脂质膜的影响，导致两个系统中脂质体收缩并形成多层脂质体。
