Asl Roya Hesari, Shojaei Shahrokh, Rad Mohammad Narimani, Asefnejad Azadeh, Jafari-Nodoushan Milad
Department of Building Engineering and Technology No. 2, Faculty of Modern Medical Technology, Azad University, Tehran Branch, Center, Tehran, Iran.
Department of Biomedical Engineering, Central Tehran Branch, Islamic Azad University, Tehran, Iran; Stem Cells Research Center, Tissue Engineering and Regenerative Medicine Institute, Central Tehran Branch, Islamic Azad University, Tehran, Iran.
Int J Biol Macromol. 2025 Aug;319(Pt 4):145680. doi: 10.1016/j.ijbiomac.2025.145680. Epub 2025 Jun 30.
Hyperglycemia and atherosclerosis caused by diabetes can significantly impair the wound healing process. An effective strategy to enhance wound healing involves the use of biomimetic dressings that enable the release of therapeutic agents and biological factors. In this study, a bilayer dressing (BL) was developed, consisting of a poloxamer 407-chitosan (P407Cs) sponge layer containing fucoidan-chitosan nanoparticles loaded with sitagliptin (F-Cs-S), and a P407Cs nanofibrous substrate for diabetic wound treatment. Chemical characterization was performed using Fourier transform infrared spectroscopy (FTIR). The results indicated that the tensile strength (4.2 ± 0.5 MPa) and elastic modulus (56 ± 3.7 MPa) of the BL dressing are sufficient for wound healing application. After 7 days in phosphate-buffered saline, the BL wound dressing showed 22.5 % weight loss and 65.2 % release of the loaded drug. The controlled release of sitagliptin (S) from the BL dressing did not exhibit cytotoxic effect and significantly enhanced angiogenesis in the chick chorioallantoic membrane. Furthermore, based on in vivo studies on the diabetic rat model, the group treated with the BL dressing exhibited the most rapid wound healing over 14 days. Consequently, the BL wound dressing can be proposed as a biomimetic dressing for the treatment of chronic diabetic wounds.
糖尿病引起的高血糖和动脉粥样硬化会显著损害伤口愈合过程。一种促进伤口愈合的有效策略是使用能够释放治疗剂和生物因子的仿生敷料。在本研究中，开发了一种双层敷料（BL），其由含有负载西他列汀的岩藻依聚糖 - 壳聚糖纳米颗粒（F - Cs - S）的泊洛沙姆407 - 壳聚糖（P407Cs）海绵层和用于糖尿病伤口治疗的P407Cs纳米纤维基质组成。使用傅里叶变换红外光谱（FTIR）进行化学表征。结果表明，BL敷料的拉伸强度（4.2±0.5MPa）和弹性模量（56±3.7MPa）足以用于伤口愈合应用。在磷酸盐缓冲盐水中放置7天后，BL伤口敷料的重量损失为22.5%，负载药物的释放率为65.2%。西他列汀（S）从BL敷料中的控释未表现出细胞毒性作用，并且显著增强了鸡胚绒毛尿囊膜中的血管生成。此外，基于对糖尿病大鼠模型的体内研究，用BL敷料治疗的组在14天内伤口愈合最快。因此，BL伤口敷料可被提议作为治疗慢性糖尿病伤口的仿生敷料。
