Prado Taiana Paola, Santos Karine Carvalho, Torres Carlos Rocha Gomes, Scaramucci Taís, Aoki Idalina Vieira, Borges Alessandra Buhler
São Paulo State University - UNESP, Institute of Science and Technology, Department of Restorative Dentistry, Avenida Engenheiro Francisco José Longo, 777, Jardim São Dimas, São José dos Campos, SP 12245-000, Brazil.
University of São Paulo - USP, School of Dentistry, Department of Restorative Dentistry, São Paulo, Brazil, Av. Prof. Lineu Prestes, 2227, Cidade Universitária, São Paulo SP 05508-000, Brazil.
J Dent. 2025 Oct;161:105944. doi: 10.1016/j.jdent.2025.105944. Epub 2025 Jun 30.
To evaluate the anti-erosive effect of solutions containing fluoride and aminomethacrylate copolymer associations on enamel and dentin, using a randomized crossover in situ model.
Bovine enamel and dentin specimens were fixed in intraoral devices. Participants (n = 12) installed the devices and rinsed with seven experimental solutions in a randomized sequence, with 2-day washout between them: Ultrapure water-C; Sodium fluoride-F (225 ppmF-); Stannous Chloride-Sn (800 ppm Sn2+); Aminomethacrylate- AA (2 %); FSn; F+AA; FSn+AA. After treatments, they retained the device for acquired pellicle maturation (30 min). Specimens were then extraorally exposed to 0.03 % HCl, pH 2.3 (3 min). This cycling was repeated six times. Calcium released from the acid solutions was measured photometrically, and mineral loss (ML) was calculated. KOHsoluble fluoride (μg/cm2) and surface microhardness (KHNred) were also measured. Data were submitted to RM-ANOVA and Tukey tests (5 %).
For mineral loss in enamel, the F+AA and FSn+AA solutions were statistically superior than F and FSn, respectively. In dentin, the F+AA solution showed the best performance (p < 0.001). The KHNred results for the enamel were: C = Sn < AA = F = FSn < F+AA = FSn+AA. The FSn groups exhibited higher concentration of fluoride adsorbed on both enamel and dentin surface (p < 0.05).
The addition of aminomethacrylate copolymer to fluoride solutions improved their efficacy in protecting the tooth against erosion in the presence of acquired pellicle, being a promising agent to be incorporated in mouthrinses.
The investigation of the anti-erosive effect of polymers and fluoride provides subsidies for identifying the most promising associations, which open new perspectives for the development of formulations capable of preventing erosion progression to more advanced stages, which requires complex and costly restorative interventions.
采用随机交叉原位模型，评估含氟化物与氨基甲基丙烯酸酯共聚物组合的溶液对牙釉质和牙本质的抗侵蚀作用。
将牛牙釉质和牙本质标本固定于口腔内装置。参与者（n = 12）佩戴该装置，并用七种实验溶液按随机顺序冲洗，每次冲洗间隔2天：超纯水-C；氟化钠-F（225 ppmF-）；氯化亚锡-Sn（800 ppm Sn2+）；氨基甲基丙烯酸酯-AA（2%）；FSn；F+AA；FSn+AA。处理后，他们保留装置以使获得性薄膜成熟（30分钟）。然后将标本口外暴露于0.03% HCl，pH 2.3（3分钟）。此循环重复六次。通过光度法测量从酸性溶液中释放的钙，并计算矿物质损失（ML）。还测量了KOH可溶性氟化物（μg/cm2）和表面显微硬度（KHNred）。数据进行重复测量方差分析和Tukey检验（5%）。
对于牙釉质中的矿物质损失，F+AA和FSn+AA溶液分别在统计学上优于F和FSn。在牙本质中，F+AA溶液表现最佳（p < 0.001）。牙釉质的KHNred结果为：C = Sn < AA = F = FSn < F+AA = FSn+AA。FSn组在牙釉质和牙本质表面吸附的氟化物浓度更高（p < 0.05）。
在含氟溶液中添加氨基甲基丙烯酸酯共聚物可提高其在存在获得性薄膜时保护牙齿免受侵蚀的功效，是一种有望纳入漱口水的制剂。
对聚合物和氟化物抗侵蚀作用的研究为确定最有前景的组合提供了依据，这为开发能够防止侵蚀进展到更严重阶段的制剂开辟了新的前景，而侵蚀进展到更严重阶段需要复杂且昂贵的修复干预措施。
