Miyamoto Emiri, Yoshihara Masato, Iyoshi Shohei, Mogi Kazumasa, Uno Kaname, Fujimoto Hiroki, Koya Yoshihiro, Kitami Kazuhisa, Yoshida Kosuke, Tamauchi Satoshi, Yokoi Akira, Yoshikawa Nobuhisa, Niimi Kaoru, Tomita Hiroyuki, Kidoya Hiroyasu, Shiraki Yukihiro, Enomoto Atsushi, Kajiyama Hiroaki
Department of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65, Tsuruma-cho, Showa-ku, Nagoya, Aichi, Japan.
Institute for Advanced Research, Nagoya University, Nagoya, Aichi, Japan.
Sci Rep. 2025 Jul 2;15(1):23196. doi: 10.1038/s41598-025-05010-3.
The impact on prognosis of neutrophil infiltration in the microenvironment of ovarian cancer remains to be elucidated. In this study, we analyzed the association between neutrophil infiltration in peritoneal metastasis and prognosis. Furthermore, we analyzed the correlation between neutrophil infiltration in peritoneal metastasis and the number of peripheral blood neutrophils, the degree of neutropenia, vascular endothelial area, and the number of stromal cells. Thirty-four specimens each of primary and metastatic advanced ovarian cancer with high-grade serous carcinoma histology were taken. Staining for MPO, CD31, and αSMA was performed on all specimens. We investigated the correlation among tissue neutrophil infiltration, prognosis, and neutrophil indicators from blood tests. Survival analysis showed that neutrophil infiltration in the primary tumor had no impact on prognosis, whereas high neutrophil infiltration in the disseminated tumor significantly shortened progression-free survival and overall survival. No correlation was found between neutrophil infiltration and the number of peripheral blood neutrophils, the degree of neutropenia, area of vascular endothelial cells, and number of stromal cells. Neutrophil infiltration into disseminated lesions of the omentum has a significant impact on prognosis. The results of the present study may provide insights for refining clinical approaches.
卵巢癌微环境中中性粒细胞浸润对预后的影响尚待阐明。在本研究中，我们分析了腹膜转移中中性粒细胞浸润与预后之间的关联。此外，我们还分析了腹膜转移中中性粒细胞浸润与外周血中性粒细胞数量、中性粒细胞减少程度、血管内皮面积以及基质细胞数量之间的相关性。采集了34例组织学类型为高级别浆液性癌的原发性和转移性晚期卵巢癌标本。对所有标本进行髓过氧化物酶（MPO）、CD31和α平滑肌肌动蛋白（αSMA）染色。我们研究了组织中性粒细胞浸润、预后以及血液检测中性粒细胞指标之间的相关性。生存分析表明，原发性肿瘤中的中性粒细胞浸润对预后无影响，而播散性肿瘤中高中性粒细胞浸润显著缩短了无进展生存期和总生存期。未发现中性粒细胞浸润与外周血中性粒细胞数量、中性粒细胞减少程度、血管内皮细胞面积以及基质细胞数量之间存在相关性。中性粒细胞浸润至大网膜播散性病变对预后有显著影响。本研究结果可能为优化临床治疗方法提供思路。
