Heidt Timo, Reiss Simon, Thielmann Julien, Weber Christian, Maier Alexander, Lottner Thomas, Cristina-Schmitz Heidi R, Bühler Timon, Chiang Diana, Jülicher Claus, Wadle Carolin, Hilgendorf Ingo, Wolf Dennis, Tumlinson Gavin, Hortells Luis, Westermann Dirk, Bock Michael, von Zur Mühlen Constantin
Department of Cardiology, Medical Center University of Freiburg, Freiburg, Germany.
Faculty of Medicine, University of Freiburg, Freiburg, Germany.
Npj Imaging. 2025 Feb 3;3(1):7. doi: 10.1038/s44303-025-00069-z.
Myocardial ischemia induces tissue injury with subsequent inflammation and recruitment of immune cells. Besides myocardial tissue characterization, magnetic resonance imaging (MRI) allows for functional assessment using molecular imaging contrast agents. Here, we assessed ischemic cardiac lesions non-invasively directly after ischemia/reperfusion (I/R) in a porcine model by advanced MRI techniques and molecular imaging, targeting the cell adhesion molecule P-selectin functionalized with microparticles of iron oxide (MPIO). We used a closed-chest model of I/R by temporary coronary balloon-occlusion, real time 3T MRI-guided coronary injection of MPIO-based contrast agents, as well as injury, edema and iron-sensitive MRI. Within the first hours after I/R, we found T1 mapping to be most sensitive for tissue injury, with no changes in edema-sensitive MRI. Intriguingly, P-selectin MPIO contrast agent selectively enhanced the ischemic area in iron-sensitive MRI. In conclusion, this approach allows for sensitive detection of early myocardial inflammation beyond traditional edema-sensitive imaging.
心肌缺血会引发组织损伤，随后出现炎症反应并募集免疫细胞。除了对心肌组织进行表征外，磁共振成像（MRI）还可使用分子成像造影剂进行功能评估。在此，我们通过先进的MRI技术和分子成像，在猪模型中缺血/再灌注（I/R）后直接对缺血性心脏病变进行无创评估，靶向用氧化铁微粒（MPIO）功能化的细胞粘附分子P-选择素。我们采用临时冠状动脉球囊闭塞的I/R闭胸模型、实时3T MRI引导下冠状动脉注射基于MPIO的造影剂，以及损伤、水肿和铁敏感MRI。在I/R后的最初几小时内，我们发现T1映射对组织损伤最为敏感，而水肿敏感MRI无变化。有趣的是，P-选择素MPIO造影剂在铁敏感MRI中选择性增强了缺血区域。总之，这种方法能够在传统的水肿敏感成像之外，灵敏地检测早期心肌炎症。
