Álvarez-López Ana Isabel, Ponce-España Eduardo, Cruz-Chamorro Ivan, Santos-Sánchez Guillermo, Bejarano Ignacio, Álvarez-Sánchez Nuria, Lardone Patricia Judith, Carrillo-Vico Antonio
Instituto de Biomedicina de Sevilla IBiS/Hospital Universitario Virgen del Rocío/CSIC/Universidad de Sevilla Seville Spain.
Departamento de Bioquímica Médica y Biología Molecular e Inmunología Facultad de Medicina Universidad de Sevilla Seville Spain.
MedComm (2020). 2025 Jul 2;6(7):e70228. doi: 10.1002/mco2.70228. eCollection 2025 Jul.
Multiple sclerosis (MS) is an autoimmune disease causing neuroinflammation and demyelination in the central nervous system (CNS). It is traditionally considered CD4 T cell-mediated, but several immune cells, such as CD8 cells, B cells, macrophages, and dendritic cells (DC) also contribute to the pathogenesis. Moreover, altered gut microbiota, including changes in specific genera, has been observed in MS patients. The murine model of MS, experimental autoimmune encephalomyelitis (EAE), is mainly carried out in C57BL/6 mice. Historically, N and J substrains have been used interchangeably, and many laboratories are not even aware of which strain they are using. Therefore, the objective of this study was to evaluate the differences between the 6J and 6N substrains subjected to myelin oligodendrocyte glycoprotein (MOG) induced EAE in the composition of neuroinflammatory cells and microbiota. 6J substrain presented a more severe EAE than the 6N substrain, accompanied by an increase in the frequency of macrophages, CD8, and B cells within the infiltrated immune cells compartment. In addition, 6J animals have a higher proinflammatory profile and a lower anti-inflammatory profile compared with the 6N substrain. Consistent with this, the differences observed in the basal microbial taxa between both substrains support the differences observed in the immunological response.
多发性硬化症（MS）是一种自身免疫性疾病，可导致中枢神经系统（CNS）发生神经炎症和脱髓鞘。传统上认为它是由CD4 T细胞介导的，但几种免疫细胞，如CD8细胞、B细胞、巨噬细胞和树突状细胞（DC）也参与了发病机制。此外，在MS患者中观察到肠道微生物群发生改变，包括特定菌属的变化。MS的小鼠模型，即实验性自身免疫性脑脊髓炎（EAE），主要在C57BL/6小鼠中进行。从历史上看，N和J亚系一直交替使用，许多实验室甚至不知道他们使用的是哪个品系。因此，本研究的目的是评估在髓鞘少突胶质细胞糖蛋白（MOG）诱导的EAE条件下，6J和6N亚系在神经炎症细胞和微生物群组成方面的差异。6J亚系表现出比6N亚系更严重的EAE，伴有浸润免疫细胞区室中巨噬细胞、CD8和B细胞频率的增加。此外，与6N亚系相比，6J动物具有更高的促炎特征和更低的抗炎特征。与此一致的是，两个亚系之间在基础微生物分类群中观察到的差异支持了在免疫反应中观察到的差异。
