Melaragno A J, Carciero R, Feingold H, Talarico L, Weintraub L, Valeri C R
Vox Sang. 1985;49(4):245-58. doi: 10.1111/j.1423-0410.1985.tb01119.x.
Platelet studies were done in healthy male volunteers and in thrombocytopenic patients. Some of the platelets used in the study were isolated by mechanical apheresis using either the Haemonetics blood processor 30, the IBM blood processor 2997 or the Fenwal CS-3000 blood processor before freezing. Other platelets were isolated from individual units of whole blood and pooled before freezing. The platelets were frozen with a 6% cryoprotectant (DMSO) in a polyvinylchloride (PVC) plastic bag or a polyolefin plastic bag at -80 degrees C in a mechanical freezer and stored for as long as 3 years. Some of the frozen platelets were transported in dry ice in polystyrene foam containers to determine whether they would be adversely affected by such treatment. Platelet recovery after freezing, thawing and washing was about 75%. In the healthy male volunteers, in vivo recovery of autologous platelets 1-2 h after transfusion was about 33%, and the life span was about 8 days. In the thrombocytopenic patients, in vivo recovery values were 50% of those from fresh platelets. The transfusion of previously frozen washed platelets reduced clinical bleeding in the thrombocytopenic patients with bleeding. There was no evidence of quality deterioration in platelets after storage at -80 degrees C for at least 2 years, as determined from in vivo recovery and in vivo survival values, nor was there any adverse effect as a result of shipment of the frozen platelets in dry ice in polystyrene foam containers from one facility to another.
在健康男性志愿者和血小板减少症患者中进行了血小板研究。研究中使用的一些血小板在冷冻前通过机械单采法分离，使用的设备有Haemonetics血液处理器30、IBM血液处理器2997或Fenwal CS - 3000血液处理器。其他血小板则从单个全血单位中分离出来并在冷冻前汇集。血小板在含有6%冷冻保护剂（二甲基亚砜）的聚氯乙烯（PVC）塑料袋或聚烯烃塑料袋中，于-80℃的机械冷冻箱中冷冻，并储存长达3年。一些冷冻血小板用干冰装在聚苯乙烯泡沫容器中运输，以确定它们是否会受到这种处理的不利影响。冷冻、解冻和洗涤后的血小板回收率约为75%。在健康男性志愿者中，输血后1 - 2小时自体血小板的体内回收率约为33%，寿命约为8天。在血小板减少症患者中，体内回收率是新鲜血小板的50%。输注先前冷冻洗涤过的血小板减少了有出血症状的血小板减少症患者的临床出血情况。根据体内回收率和体内存活值判断，在-80℃储存至少2年后，血小板没有质量恶化的迹象，将冷冻血小板用干冰装在聚苯乙烯泡沫容器中从一个机构运输到另一个机构也没有产生任何不良影响。
