Spector J I, Yarmala J A, Marchionni L D, Emerson C P, Valeri C R
Transfusion. 1977 Jan-Feb;17(1):8-15. doi: 10.1046/j.1537-2995.1977.17177128894.x.
Each of 15 healthy male volunteers was treated with 650 mg of aspirin 24 hours before the autologous transfusion of one unit of freeze-preserved platelets. Freeze-thaw-wash recovery values in vitro, viability and function in vivo, and the bleeding time and platelet aggregation response were measured. The platelets were frozen with 4 or 5 per cent dimethylsulfoxide (DMSO) at an overall rate of 2 to 3 C per minute and were stored at -80 C in a mechanical freezer for up to eight months. They were washed by dilution/centrifugation. The mean recovery in vitro of platelets frozen with 4 per cent DMSO was 76+/-16%; the value was 64+/-16% for platelets frozen with 5% DMSO. The mean in vivo 51Cr recovery of autologous platelets frozen with 4% DMSO was 34+/-6%, and for platelets frozen with 5% DMSO it was 33+/-7%. In both groups the platelet lifespan was normal. There was a significant reduction in bleeding time after the transfusion of a single unit of autologous platelets preserved with either 4 or 5% DMSO, but no improvement in the aspirin-induced platelet aggregation pattern.
15名健康男性志愿者在自体输注1单位冷冻保存的血小板前24小时，均接受650毫克阿司匹林治疗。测量了体外冻融洗涤回收率、体内活力和功能，以及出血时间和血小板聚集反应。血小板用4%或5%的二甲亚砜（DMSO）以每分钟2至3摄氏度的总体速率冷冻，并在-80摄氏度的机械冷冻箱中储存长达8个月。通过稀释/离心进行洗涤。用4% DMSO冷冻的血小板体外平均回收率为76±16%；用5% DMSO冷冻的血小板该值为64±16%。用4% DMSO冷冻的自体血小板体内51Cr平均回收率为34±6%，用5% DMSO冷冻的血小板为33±7%。两组的血小板寿命均正常。输注用4%或5% DMSO保存的单单位自体血小板后，出血时间显著缩短，但阿司匹林诱导的血小板聚集模式无改善。
