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[通过聚乙烯薄膜上的固相放射免疫分析检测乙肝表面抗原]

[Detection of HBsAg by solid-phase radioimmunological analysis on polyethylene film].

作者信息

Prokudina E N, Semenova N P, Viazov S O, Anan'ev V A, Zhdanov V M

出版信息

Vopr Virusol. 1985 Jul-Aug;30(4):413-6.

PMID:4060698
Abstract

A method of solid-phase radioimmunoassay on polyethylene film has been developed for the detection of HBsAg in human sera. The method records the results autoradiographically, is very capacious, demonstrative and documentary. It has been demonstrated that for at least one month the antibodies previously immobilized on the film retain their capacity to bind with HBsAg that allows timely preparation of the film with immobilized antibody for future analyses. Detection of HBsAg on the film was shown to be specific. The reasons are given for large-scale application of the method in the laboratories not equipped with special radiometric apparatuses.

摘要

已开发出一种在聚乙烯薄膜上进行固相放射免疫测定的方法,用于检测人血清中的乙肝表面抗原(HBsAg)。该方法通过放射自显影记录结果,容量大、具有示范性且有记录性。已证明,预先固定在薄膜上的抗体至少在一个月内仍保留其与HBsAg结合的能力,这使得能够及时制备带有固定抗体的薄膜以备将来分析之用。结果表明,在薄膜上检测HBsAg具有特异性。文中给出了该方法可在未配备特殊辐射测量仪器的实验室中大规模应用的原因。

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