Shchegolev A S, Kushch A A, Momot A M, Shumaĭ E P, Skoblov Iu S
Vopr Virusol. 1987 Sep-Oct;32(5):544-7.
The sensitivity of test systems for radioimmunological detection of HBsAg prepared on the basis of polyclonal immunoglobulins, affinity polyclonal antibody (PCA) and monoclonal antibody (MCA) was compared. MCA ZhAK 22 and ZhAK 12 interacting with two different HBsAg determinants: general-type "a" and subtype "y", respectively, were used. The solid phase was sensitized with MCA of each clone or a mixture of MCA in concentration ratios of 1:1, 1:2, and 2:1. After sensitization of the solid phase and addition of HBsAg, 125I-labeled affinity PCA were added (in all tests). The radioimmunological test-system based on MCA ZhAK 22 and ZhAK 12 adsorbed on the solid phase at a 2:1 ratio was found the most sensitive: 0.5 ng, i.e. 4 times as high as that of the system prepared on the basis of PCA.
比较了基于多克隆免疫球蛋白、亲和多克隆抗体（PCA）和单克隆抗体（MCA）制备的用于放射免疫检测乙肝表面抗原（HBsAg）的检测系统的灵敏度。使用了分别与两种不同的HBsAg决定簇相互作用的MCA ZhAK 22和ZhAK 12：分别为通用型“a”和亚型“y”。固相用每个克隆的MCA或浓度比为1:1、1:2和2:1的MCA混合物进行致敏。在固相致敏并添加HBsAg后，添加125I标记的亲和PCA（在所有测试中）。发现基于以2:1比例吸附在固相上的MCA ZhAK 22和ZhAK 12的放射免疫检测系统最灵敏：0.5纳克，即比基于PCA制备的系统高4倍。
