Zhao Jing, Li Yanhong, Ling Yingying, Wu Tong, Wu Yinlan, Tan Chunyu, Cheng Lu, Huang Deying, Liu Yi, Zhang Yong
Department of Rheumatology and Immunology, Laboratory of Rheumatology and Immunology, Institutes for Systems Genetics, West China Hospital, Sichuan University, Chengdu, China.
Front Immunol. 2025 Jun 18;16:1538219. doi: 10.3389/fimmu.2025.1538219. eCollection 2025.
Anti-synthetase syndrome (ASS) is a subtype of idiopathic inflammatory myopathy (IIM) characterized by characteristic rash, myositis, and interstitial lung disease (ILD). The etiology of ASS is unknown, and patients have a poor quality of life and are prone to pulmonary infection. Recent studies have elucidated the potential role of abnormal glycosylation of immunoglobulin G (IgG) in the pathogenesis of autoimmune diseases. However, the pattern of patient-specific IgG N-glycosylation in ASS has not been fully elucidated.
the GlycoQuant method was used to quantify the intact N-glycopeptides of IgG from 30 ASS patients and 30 healthy controls (HCs).
Thirteen differentially expressed intact N-glycopeptides were identified (p<0.05). Notably, we observed increased fucosylation (p<0.0001) and decreased N-acetylneuraminic acid (p<0.05) in ASS patients. In addition, specific glycosylation patterns correlated with lung function parameters. Our study revealed the IgG glycosylation profile in ASS patients and provided a valuable reference for further investigation of its potential diagnostic and prognostic applications.
抗合成酶综合征（ASS）是特发性炎性肌病（IIM）的一种亚型，其特征为典型皮疹、肌炎和间质性肺疾病（ILD）。ASS的病因尚不清楚，患者生活质量较差，且易发生肺部感染。最近的研究阐明了免疫球蛋白G（IgG）异常糖基化在自身免疫性疾病发病机制中的潜在作用。然而，ASS中患者特异性IgG N-糖基化模式尚未完全阐明。
采用GlycoQuant方法对30例ASS患者和30例健康对照（HCs）的IgG完整N-糖肽进行定量分析。
鉴定出13种差异表达的完整N-糖肽（p<0.05）。值得注意的是，我们观察到ASS患者岩藻糖基化增加（p<0.0001），N-乙酰神经氨酸减少（p<0.05）。此外，特定的糖基化模式与肺功能参数相关。我们的研究揭示了ASS患者的IgG糖基化谱，为进一步研究其潜在的诊断和预后应用提供了有价值的参考。
