Park Jonggwan, Lee Seulbi, Kang Da Dam, Oh Jun Hee, Kim Hyeongsun, Park Yoonkyung
Department of Bioinformatics, Kongju National University, Kongju, 32588, Republic of Korea.
Department of Biomedical Science, Chosun University, Gwangju, 61452, Republic of Korea.
AMB Express. 2025 Jul 3;15(1):99. doi: 10.1186/s13568-025-01908-3.
The increasing prevalence of antimicrobial resistance underscores the urgent need for alternative treatments. Antimicrobial peptides (AMPs) are considered viable substitutes for conventional antibiotics due to their broad-spectrum activity and unique mechanisms of action. AMPs are an integral component of the innate immune system in invertebrates. Mastoparan-S, a 14-amino acid AMP originally identified in the praying mantis Sphodromantis viridis reportedly exhibits antimicrobial activity against gram-positive and gram-negative bacteria, as well as fungi. In this study, the antibacterial activity and mechanism of action of chemically-synthesized mastoparan-S are evaluated against Staphylococcus aureus and Pseudomonas aeruginosa. Mastoparan-S exhibits potent antibacterial activity against S. aureus and P. aeruginosa, without inducing cytotoxicity or hemolytic activity in RAW264.7 or HaCaT cells. Mastoparan-S effectively inhibits biofilm formation by S. aureus and P. aeruginosa. Mechanistically, mastopran-S kills bacteria by disrupting the bacterial membrane. Collectively, these findings suggest that mastoparan-S is a potential therapeutic agent for treating S. aureus and P. aeruginosa infections.
抗菌耐药性的日益普遍凸显了对替代治疗方法的迫切需求。抗菌肽（AMPs）因其广谱活性和独特的作用机制而被认为是传统抗生素的可行替代品。AMPs是无脊椎动物先天免疫系统的一个组成部分。马蜂毒素-S是一种最初在绿巨螳螂中发现的由14个氨基酸组成的抗菌肽，据报道它对革兰氏阳性菌、革兰氏阴性菌以及真菌都具有抗菌活性。在本研究中，评估了化学合成的马蜂毒素-S对金黄色葡萄球菌和铜绿假单胞菌的抗菌活性及作用机制。马蜂毒素-S对金黄色葡萄球菌和铜绿假单胞菌表现出强大的抗菌活性，且在RAW264.7或HaCaT细胞中不诱导细胞毒性或溶血活性。马蜂毒素-S能有效抑制金黄色葡萄球菌和铜绿假单胞菌的生物膜形成。从机制上讲，马蜂毒素-S通过破坏细菌膜来杀死细菌。总体而言，这些发现表明马蜂毒素-S是治疗金黄色葡萄球菌和铜绿假单胞菌感染的潜在治疗剂。
