Yamada A, Brown L E, Webster R G
Vaccine. 1985 Sep;3(3 Suppl):195-8. doi: 10.1016/0264-410x(85)90102-1.
Antigenic analysis of human and avian H2 influenza virus was carried out with monoclonal antibodies to the HA molecules of H2 influenza viruses isolated in the early stage of an H2 pandemic. The study revealed antigenic differences between inhibitor sensitive (Japan+/57, RI+57) and inhibitor resistant strains (Japan-/57, Ri-/57). This indicates that the receptor-binding specificity of the haemagglutinin can markedly influence the antigenic analysis obtained with monoclonal antibodies in HI test. Minor antigenic differences (microheterogeneity) could be detected between different H2 influenza viruses isolated in 1957. Minor antigenic variation continued in the H2 viruses until 1961, but significant antigenic drift occurred in 1962 so that viruses isolated after that date reacted with few monoclonal antibodies. Analysis of avian H2 influenza viruses suggested antigenic differences between the different avian H2 haemagglutinin, but no correlation between the year of isolation and the progressive antigenic drift similar to that seen in the human strains was found.
利用针对H2大流行早期分离出的H2流感病毒HA分子的单克隆抗体，对人源和禽源H2流感病毒进行了抗原分析。研究揭示了抑制剂敏感株（日本+/57、RI+57）和抑制剂耐药株（日本-/57、Ri-/57）之间的抗原差异。这表明血凝素的受体结合特异性可显著影响HI试验中用单克隆抗体获得的抗原分析结果。1957年分离出的不同H2流感病毒之间可检测到微小的抗原差异（微异质性）。H2病毒中微小的抗原变异一直持续到1961年，但在1962年发生了显著的抗原漂移，以至于该日期之后分离出的病毒与很少的单克隆抗体发生反应。对禽源H2流感病毒的分析表明，不同禽源H2血凝素之间存在抗原差异，但未发现分离年份与类似于人源毒株中所见的渐进性抗原漂移之间存在相关性。
