Wang Yuda, Shen Jianyong, Liu Guangtao, Jiang Ziqi, Liu Hang, Yu Tao, Ying Zhuoxin, Lin Junfen, Liu Yan
Huzhou Center for Disease Control and Prevention, Huzhou, Zhejiang, China.
Zhejiang Province Infectious Disease Surveillance and Early Warning Professional Backbone Training Project, Hangzhou, Zhejiang, China.
PLoS One. 2025 Jul 3;20(7):e0326382. doi: 10.1371/journal.pone.0326382. eCollection 2025.
Avian influenza is an infectious disease of birds caused by the influenza A virus, which can infect a variety of domestic,wild birds and even cross the species barrier and infect humans.To understand the contamination of avian influenza virus in the external environment of poultry in Huzhou City from 2017 to 2023 and to assess the risk of human infection with avian influenza.
A total of 3,400 environmental specimens from five types of venues in Huzhou City were collected and tested for influenza A virus nucleic acid using fluorescent reverse transcription polymerase chain reaction (RT-PCR).
From 2017 to 2023, with 15.44% overall positive rate of influenza A virus. The predominant subtype of avian influenza virus was H9 (accounting for 54.67%). The peak of positive influenza virus detection rates occurred in winter and spring seasons every year. The venue with highest positive rate was poultry slaughtering and processing plants (41.83%), followed by urban and rural live poultry markets (35.48%); among all types of specimens, the highest positive rate was detected in swab specimens from the surfaces of poultry slaughtering or display tables (47.37%), followed by wastewater from poultry washing (45.83%), and surfaces of cages (27.65%).
The contamination of avian influenza virus in the poultry environment in Huzhou City is relatively severe, with diverse subtypes. There is a potential risk of human infection with avian influenza virus, and real-time monitoring of avian influenza virus in the poultry environment needs to be strengthened.
禽流感是由甲型流感病毒引起的禽类传染病，可感染多种家禽、野鸟，甚至跨越物种屏障感染人类。为了解湖州市2017年至2023年家禽养殖外部环境中禽流感病毒的污染情况，并评估人类感染禽流感的风险。
共收集湖州市5类场所的3400份环境标本，采用荧光逆转录聚合酶链反应（RT-PCR）检测甲型流感病毒核酸。
2017年至2023年，甲型流感病毒总体阳性率为15.44%。禽流感病毒的主要亚型为H9（占54.67%）。每年流感病毒检测阳性率的高峰出现在冬春季节。阳性率最高的场所是家禽屠宰加工厂（41.83%），其次是城乡活禽市场（35.48%）；在所有类型的标本中，家禽屠宰或展示台面拭子标本的阳性率最高（47.37%），其次是家禽清洗废水（45.83%）和笼具表面（27.65%）。
湖州市家禽养殖环境中禽流感病毒污染较为严重，亚型多样。存在人类感染禽流感病毒的潜在风险，需加强对家禽养殖环境中禽流感病毒的实时监测。
