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[Wistar大鼠离体灌注肝脏对标记油酸和甘油的摄取与掺入]

[Uptake and incorporation of labeled oleic acid and glycerol by the isolated and perfused liver of the Wistar rat].

作者信息

Chanussot F, Debry G

出版信息

C R Acad Hebd Seances Acad Sci D. 1977 May 2;284(17):1681-4.

PMID:406089
Abstract

After perfusion by oleic acid (9-10(-3)H) and glycerol (1(-14)C) previously starved Wistar rats, the synthesis of hepatic TG and PL follows the two following different method: -- during the first minutes of perfusions, the most important method synthesis of TG and especially of PL is a de novo synthesis utilizing glycerol and the exogenous AG. The TG synthesized are 18:1 18:1 18:1 and 16:0 18:1 18:2 the PL synthesized are LP, AP and LPC; -- during perfusions of long duration (30, 60, 120 min.), the major method of synthesis of TG and PL is an active exchange of AG of the endogenous glycerolipids. The TG synthesized are 16:0 18:1 18:1 and 16:0 18:1 18:2 the PL synthesized are PE and PC.

摘要

在用油酸(9 - 10(-3)H)和甘油(1(-14)C)灌注先前饥饿的Wistar大鼠后,肝脏甘油三酯(TG)和磷脂(PL)的合成遵循以下两种不同方法:——在灌注的最初几分钟内,TG尤其是PL的最重要合成方法是利用甘油和外源性酰基甘油(AG)的从头合成。合成的TG为18:1 18:1 18:1和16:0 18:1 18:2,合成的PL为溶血磷脂(LP)、酰基磷脂(AP)和溶血磷脂酰胆碱(LPC);——在长时间灌注(30、60、120分钟)期间,TG和PL合成的主要方法是内源性甘油脂质的AG的活跃交换。合成的TG为16:0 18:1 18:1和16:0 18:1 18:2,合成的PL为磷脂酰乙醇胺(PE)和磷脂酰胆碱(PC)。

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