[用标记油酸和甘油灌注的离体Wistar大鼠肝脏释放合成脂质]

[Release of synthesized lipids by isolated Wistar rat liver perfused by labelled oleic acid and glycerol].

作者信息

Chanussot F, Derby G

出版信息

C R Acad Hebd Seances Acad Sci D. 1977 Sep 26;285(6):697-700.

Abstract

After perfusions with oleic acid (9, 10-3H) and glycerol (1-14C) of isolated livers from wistar Rats, previously subjected to fasting, the study of the TG and PL synthesized by the liver and released in the circulation gives of evidence similar ways of metabolising TG and PL. Two liver pools are present: -- a storage pool, built with slow exchanges of AG of endogenous glycerolipids. In our experiments, the TG: 16 : 0 18 : 1 18: 2 and 16 : 0 18 : 1 18 : 1 and the PL: PE and PC are the glycerolipids of liver constitution: -- a pool of liver secretion. TG and PL are formed according to a rapid de novo synthesis using preferentially the exogenous substrates. They are released in the circulation. In our experiments, the TG secreted are the TG 18 : 1 18 : 1 18 : 1 and 16 : 0 18 : 1 18 : 2, the PL are the PE, AP and LPC.

摘要

在用油酸（9,10 - ³H）和甘油（1 - ¹⁴C）灌注先前禁食的Wistar大鼠的离体肝脏后，对肝脏合成并释放到循环中的甘油三酯（TG）和磷脂（PL）的研究给出了TG和PL代谢方式相似的证据。存在两个肝池：——一个储存池，由内源性甘油脂质的酰基甘油（AG）缓慢交换构成。在我们的实验中，TG：16 : 0 18 : 1 18: 2和16 : 0 18 : 1 18 : 1以及PL：磷脂酰乙醇胺（PE）和磷脂酰胆碱（PC）是肝脏构成的甘油脂质；——一个肝脏分泌池。TG和PL通过优先使用外源性底物的快速从头合成而形成。它们被释放到循环中。在我们的实验中，分泌的TG是TG 18 : 1 18 : 1 18 : 1和16 : 0 18 : 1 18 : 2，PL是PE、溶血磷脂酸（AP）和溶血磷脂酰胆碱（LPC）。