Dumitru Ionut, Paterlini Marta, Zamboni Margherita, Ziegenhain Christoph, Giatrellis Sarantis, Saghaleyni Rasool, Björklund Åsa, Alkass Kanar, Tata Mathew, Druid Henrik, Sandberg Rickard, Frisén Jonas
Department of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
Dept of Biology and Biological Engineering, National Bioinformatics Infrastructure Sweden, Science for Life Laboratory, Chalmers University of Technology, Göteborg, Sweden.
Science. 2025 Jul 3;389(6755):58-63. doi: 10.1126/science.adu9575.
Continuous adult hippocampal neurogenesis is involved in memory formation and mood regulation but is challenging to study in humans. Difficulties finding proliferating progenitor cells called into question whether and how new neurons may be generated. We analyzed the human hippocampus from birth through adulthood by single-nucleus RNA sequencing. We identified all neural progenitor cell stages in early childhood. In adults, using antibodies against the proliferation marker Ki67 and machine learning algorithms, we found proliferating neural progenitor cells. Furthermore, transcriptomic data showed that neural progenitors were localized within the dentate gyrus. The results contribute to understanding neurogenesis in adult humans.
成年海马体的持续神经发生参与记忆形成和情绪调节，但在人类中进行研究具有挑战性。难以找到增殖的祖细胞，这引发了关于新神经元是否以及如何产生的疑问。我们通过单核RNA测序分析了从出生到成年的人类海马体。我们在幼儿期识别出了所有神经祖细胞阶段。在成年人中，使用针对增殖标志物Ki67的抗体和机器学习算法，我们发现了增殖的神经祖细胞。此外，转录组数据表明神经祖细胞位于齿状回内。这些结果有助于理解成年人类的神经发生。
