Desalegn Samuel, Abaya Galana, G'Yesus Tarekegn, Admasu Dawit, Mussema Abdulhakim
Department of Biotechnology, College of Natural and Computational Science, Wachemo University, Hosanna, Ethiopia.
Department of Medical Laboratory Science, College of Medicine and Health Science, Wachemo University, Hosanna, Ethiopia.
IJID Reg. 2025 Mar 3;15:100617. doi: 10.1016/j.ijregi.2025.100617. eCollection 2025 Jun.
Intestinal parasites, along with and , are significant causes of acute diarrhea in resource-limited countries, posing challenges for health authorities. This study aimed to assess the prevalence and risk factors for these pathogens among food handlers at Wachemo University students' food service facility.
A cross-sectional study was conducted from June to November 2022, involving 390 food handlers selected through random sampling. Data were collected via a semi-structured questionnaire, and stool samples were analyzed for , and intestinal parasites using microscopic examination and selective culture media. species were subsequently identified using standard bacteriological protocols. Antimicrobial susceptibility was evaluated using the Kirby-Bauer disk diffusion method and data were analyzed by using SPSS version 20.
Prevalence rates were 4.6% for spp. and 2.1% for spp. Bacterial isolates showed high sensitivity to gentamicin (92.3%) and ciprofloxacin (88.5%), but 100% and 61.5% resistance to ampicillin and ceftriaxone, respectively. Additionally, 32.3% of samples tested positive for intestinal parasites, with / being the most common (17.7%). Poor hygiene practices such as untrimmed nails and inadequate handwashing, were linked to these infections.
This study highlights the health risks posed by these pathogens among food handlers. It highlights the need for health education, regular check-ups, and rational antibiotic use at Wachemo University students' food service facility.
在资源有限的国家，肠道寄生虫以及[此处原文缺失两种病原体名称]是急性腹泻的重要病因，给卫生当局带来了挑战。本研究旨在评估瓦切莫大学学生餐饮服务设施中食品处理人员感染这些病原体的患病率及风险因素。
于2022年6月至11月进行了一项横断面研究，通过随机抽样选取了390名食品处理人员。通过半结构化问卷收集数据，并使用显微镜检查和选择性培养基对粪便样本进行[此处原文缺失两种病原体名称]及肠道寄生虫分析。随后使用标准细菌学方案鉴定[此处原文缺失细菌名称]菌种。采用 Kirby-Bauer 纸片扩散法评估抗菌药物敏感性，并使用SPSS 20版软件对数据进行分析。
[此处原文缺失细菌名称]菌种的患病率为4.6%，[此处原文缺失细菌名称]菌种的患病率为2.1%。细菌分离株对庆大霉素（92.3%）和环丙沙星（88.5%）表现出高敏感性，但对氨苄西林和头孢曲松的耐药率分别为100%和61.5%。此外，32.3%的样本肠道寄生虫检测呈阳性，其中[此处原文缺失寄生虫名称]/[此处原文缺失寄生虫名称]最为常见（17.7%）。指甲未修剪和洗手不充分等不良卫生习惯与这些感染有关。
本研究强调了这些病原体在食品处理人员中造成的健康风险。突出了在瓦切莫大学学生餐饮服务设施开展健康教育、定期体检和合理使用抗生素的必要性。
