Xi Yitao, Akram Sawera, Yang Xinyi, Hu Beibei, Saddique Muhammad Abu Bakar, Guan Ge, Tian Donglan, Luo Xiumei, Ren Maozhi
Institute of Urban Agriculture, Chinese Academy of Agricultural Sciences, Chengdu Agricultural Science and Technology Center, Chengdu 610000, China.
School of Agricultural Science of Zhengzhou University, Zhengzhou, Henan 450000, China.
J Agric Food Chem. 2025 Jul 16;73(28):17344-17357. doi: 10.1021/acs.jafc.5c02537. Epub 2025 Jul 4.
Chemical pesticides have traditionally been a primary strategy for controlling plant diseases. With the mounting issues in disease control arising from the prolonged use of pesticides, the three ″R″ issues (resistance, residues, and resurgence) and the three ″risks″ (disability, teratogenicity, and mutagenicity) have become more prominent, significantly hindering agricultural development. There is an urgent need to develop sustainable and environmentally friendly approaches to prevent and control plant diseases. RNA pesticides are poised to lead a green revolution in agriculture, and spray-induced gene silencing (SIGS), depending on dsRNA and nanotechnology, is a novel approach offering efficient and targeted strategies for sustainable management of plant pathogens. The technology avoids the potential ecological risks associated with genetically modified crops. This review outlines the recent progress in understanding the molecular mechanisms of dsRNA silencing in plant pathogens and focuses on the advancements in SIGS using nanotechnology for managing plant diseases. The combination of SIGS with nanopesticide will provide a dual effect on disease control.
传统上，化学农药一直是控制植物病害的主要策略。随着长期使用农药导致的病害控制问题日益增多，“三R”问题（抗性、残留和再增猖獗）以及“三致风险”（致畸性、致突变性和致癌性）变得更加突出，严重阻碍了农业发展。迫切需要开发可持续且环境友好的方法来预防和控制植物病害。RNA农药有望引领农业绿色革命，而基于双链RNA（dsRNA）和纳米技术的喷雾诱导基因沉默（SIGS）是一种为植物病原体可持续管理提供高效且靶向策略的新方法。该技术避免了与转基因作物相关的潜在生态风险。本文综述概述了在理解植物病原体中dsRNA沉默分子机制方面的最新进展，并重点介绍了利用纳米技术进行SIGS以管理植物病害的进展。SIGS与纳米农药的结合将对病害控制产生双重效果。
