Amirizadeh Shiva, Pasbakhsh Parichehr, Omairi Saleh, Ragerdi Kashani Iraj, Hashemi Maedeh, Zarini Davood, Asadi-Golshan Reza
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, 1461884513, Iran.
Department of Human Anatomy, College of Medicine, Wasit University, Kut, Iraq.
J Mol Histol. 2025 Jul 5;56(4):217. doi: 10.1007/s10735-025-10509-0.
Multiple sclerosis (MS) leads to histological changes, which in turn result in functional deficits. Studies have reported that bone marrow mesenchymal stem cells (BMSCs) have therapeutic benefits in MS. In addition, sleep deprivation affects therapeutic potential of BMSCs. This research aimed to evaluate the effects of BMSCs compared to sleep-deprived BMSCs (SD-BMSCs) on the motor behavior and histological recovery in MS. The mice were divided into four groups receiving cuprizone for five weeks, with or without intranasal administration of cells (BMSCs or SD-BMSCs) after five weeks. After a two-week recovery period, balance beam and pole tests were performed to assess motor performance, followed by estimation of histological parameters using stereological methods. Mice that received BMSCs scored significantly better than the SD-BMSC group in balance beam and pole tests (P < 0.05). The total cerebellar volume, cortex, and white matter volumes, as well as the total number of Purkinje cells in mice that received BMSCs, were significantly higher compared to those in the SD-BMSC group (P < 0.05). Intranasal administration of BMSCs leads to significantly improved motor behavior and histological regeneration of the cerebellum, including cortex and white matter as well as Purkinje cells compared to SD-BMSCs in a cuprizone model of multiple sclerosis.
多发性硬化症（MS）会导致组织学变化，进而导致功能缺陷。研究报告称，骨髓间充质干细胞（BMSCs）对MS具有治疗作用。此外，睡眠剥夺会影响BMSCs的治疗潜力。本研究旨在评估BMSCs与睡眠剥夺的BMSCs（SD-BMSCs）相比，对MS小鼠运动行为和组织学恢复的影响。将小鼠分为四组，给予铜螯合剂五周，五周后进行或不进行鼻内细胞给药（BMSCs或SD-BMSCs）。经过两周的恢复期后，进行平衡木和杆试验以评估运动性能，随后使用体视学方法估计组织学参数。在平衡木和杆试验中，接受BMSCs的小鼠得分明显高于SD-BMSC组（P < 0.05）。与SD-BMSC组相比，接受BMSCs的小鼠的小脑总体积、皮质和白质体积以及浦肯野细胞总数明显更高（P < 0.05）。在多发性硬化症的铜螯合剂模型中，与SD-BMSCs相比，鼻内给予BMSCs可显著改善运动行为，并促进小脑的组织学再生，包括皮质、白质以及浦肯野细胞。
