Wolff H, Schill W B
Andrologia. 1985 Sep-Oct;17(5):426-34. doi: 10.1111/j.1439-0272.1985.tb01033.x.
To find optimal test conditions of an indirect enzyme-linked immunosorbent assay (ELISA) for the detection of antisperm antibodies (ASA), several variations (solid phases, antigen load, incubations etc.) have been performed. In contrast to the so far used polystyrene microtiter plates, which have to be coated with spermatozoa by the help of glutaraldehyde, we applied positively charged PVC microtiter plates being able to adsorb unfixed spermatozoa and thus avoiding alteration of spermatozoal antigens by glutaraldehyde. The ELISA proved to be more sensitive than conventional methods, e.g. the GAT, the SIT and the TAT, and also more effective since the technique allows screening of more than one hundred test samples for ASA within one day. First results are presented evaluating sera and seminal plasma of andrological and dermatological patients and ten known fertile men for ASA by the described ELISA technique.
为了找到用于检测抗精子抗体（ASA）的间接酶联免疫吸附测定（ELISA）的最佳测试条件，已经进行了多种变化（固相、抗原负载、孵育等）。与迄今为止使用的必须借助戊二醛用精子包被的聚苯乙烯微量滴定板不同，我们应用了带正电荷的PVC微量滴定板，它能够吸附未固定的精子，从而避免戊二醛对精子抗原的改变。ELISA被证明比传统方法（如GAT、SIT和TAT）更灵敏，并且也更有效，因为该技术允许在一天内对一百多个测试样品进行ASA筛查。本文展示了通过所述ELISA技术评估男科和皮肤科患者以及十名已知可育男性的血清和精浆中ASA的初步结果。
