A comparison of enzyme-linked immunosorbent assay (ELISA) with sperm immobilization test (SIT) in detection of antisperm antibodies in sera of infertile women.

In the present study, an enzyme-linked immunosorbent assay (ELISA) for antisperm antibodies was compared with a traditional standard sperm immobilization test (SIT) in sera from 158 infertile women in order to evaluate their clinical usefulness for various causes of infertility. Ten pregnant and unmarried women were used as the control group. There was a higher incidence rate by ELISA (13.9%) compared with that by SIT (10.1%) after examination of overall samples, although the difference was not statistically significant. The unexplained infertility group exhibited the highest incidence among the various causes of infertility in both of these two methods. Fifteen (93.8%) of 16 patients with positive SIT showed positive reaction in ELISA method, indicating that a close correlation exists between the two methods. The positive predictive value (54.5%) of ELISA was significantly (p < 0.05) lower than that (87.5%) of SIT, as evaluated with the results of postcoital test (PCT) performed at periovulatory phase. This suggests that the ELISA method for antisperm antibodies is less specific than SIT in view of the PCT results. Nevertheless, no fresh sperms are not required for the ELISA examination. Thus, it seems that the ELISA method may provide a convenient and practical means for mass screening in detecting antisperm antibodies in clinical practice.