Waud B E, Amaki Y, Waud D R
Anesth Analg. 1985 Dec;64(12):1178-82.
The effect of immobilization on subsequent sensitivity of limb muscles to d-tubocurarine was examined in a guinea pig model. A foreleg was immobilized in a cast for 1-4 weeks. At the end of this period, isolated nerve-lumbrical muscle preparations were set up and sensitivity to d-tubocurarine assayed. Immobilization increased the ED50 of the drug not only in muscles from the limb in the cast but also those from contralateral forelimbs and from hindlimbs. The increase in ED50 was not accompanied by atrophy. In a parallel series of experiments measuring the effect of d-tubocurarine on depolarization by carbachol, the apparent d-tubocurarine-receptor dissociation constant was determined. Immobilization produced no change in this parameter. Because immobilization of one extremity produced sensitivity changes in others, the anesthesiologist must be careful not to assume that monitoring from any limb will yield normal values in a patient with partial immobility.
在豚鼠模型中研究了肢体固定对随后肢体肌肉对d - 筒箭毒碱敏感性的影响。将一只前肢用石膏固定1至4周。在此期间结束时，制备分离的神经 - 蚓状肌标本并测定对d - 筒箭毒碱的敏感性。固定不仅增加了来自石膏固定肢体肌肉中该药物的半数有效量（ED50），还增加了来自对侧前肢和后肢肌肉中的ED50。ED50的增加并未伴有萎缩。在一系列平行实验中测量d - 筒箭毒碱对卡巴胆碱去极化的影响时，测定了表观d - 筒箭毒碱 - 受体解离常数。固定对此参数没有影响。由于一个肢体的固定会导致其他肢体出现敏感性变化，因此麻醉医生必须注意，对于部分肢体固定的患者，不要认为从任何肢体进行监测都会得到正常数值。
