苯乙烯-马来酸-百里醌纳米胶束的制备及其在三阴性乳腺癌模型中的体内外应用
Styrene-maleic acid-thymoquinone nanomicelles preparation, and their in vitro and in vivo application in a model of TNBC.
作者信息
Alhamoud Marah Abdulhadi, Hamidaddin Fatima Alhassan, Haider Mohamed, Pittalà Valeria, Greish Khaled
机构信息
Medical Intern, Imam Abdulrahman bin Faisal University, Dammam, Saudi Arabia.
Medical Intern, King Saud University Medical City, Riyadh, Saudi Arabia.
出版信息
Ther Deliv. 2025 Aug;16(8):745-754. doi: 10.1080/20415990.2025.2527577. Epub 2025 Jul 6.
PURPOSE
To develop and characterize a micellar system, SMA-TQ (styrene-maleic acid-thymoquinone), for the potential management of triple-negative breast cancer (TNBC).
METHODS
SMA synthesis and TQ encapsulation in SMA are described. The loading of TQ in SMA-TQ was established at 20%. The size of SMA-TQ micelle as determined by DLS was in average of 155.2 nm with a polydispersity index of 0.350. In vitro testing was carried out in 4T1 TNBC cell line, and in vivo testing in mice model of TNBC.
RESULTS
In vitro experiments revealed an added effect of SMA-TQ and doxorubicin (Doxo) at doses of 1-3 mM). In vivo studies showed that the combination of SMA-TQ and Doxo had the least mean tumor growth (509.6%) compared to other treatments.
CONCLUSION
Obtained results collectively suggest that the combination of SMA-TQ and Doxo has cumulative effects in inhibiting tumor growth, emphasizing the significance of combination therapies to enhance anticancer effectiveness. This work reveals SMA-TQ micellar system as complementary therapeutic option in TNBC.
目的
开发并表征一种胶束系统SMA-TQ（苯乙烯-马来酸-百里醌），用于三阴性乳腺癌（TNBC）的潜在治疗。
方法
描述了SMA的合成以及TQ在SMA中的包封。SMA-TQ中TQ的负载量确定为20%。通过动态光散射（DLS）测定，SMA-TQ胶束的平均粒径为155.2 nm，多分散指数为0.350。在4T1 TNBC细胞系中进行了体外测试，并在TNBC小鼠模型中进行了体内测试。
结果
体外实验显示，在1-3 mM剂量下，SMA-TQ和阿霉素（Doxo）具有相加作用。体内研究表明，与其他治疗方法相比，SMA-TQ和Doxo联合使用时肿瘤平均生长率最低（509.6%）。
结论
所得结果共同表明，SMA-TQ和Doxo联合使用在抑制肿瘤生长方面具有累积效应，强调了联合治疗提高抗癌效果的重要性。这项工作揭示了SMA-TQ胶束系统可作为TNBC的一种补充治疗选择。
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