Farkas-Himsley H, Pagel A
Infect Immun. 1977 Apr;16(1):12-9. doi: 10.1128/iai.16.1.12-19.1977.
A rapid and reproducible method of bacteriocin typing is described based on leakage of ultraviolet light-absorbing material (UVAM), detectable in supernatants of bacteriocin-sensitive cultures, by means of a spectrophotometer. The prerequisites for reproducible results, with nonsignificant fluctuations in standard error of the mean, are: a set of standardized bacteriocins, produced under defined conditions and of determined strength. These must interact with the unknown bacterial culture in suspension and at a given ratio in order to achieve an optimal multiplicity of interaction. Pyocin and colicin typing by the "scrape and streak" technique of Gillies (J. Hyg. 62:1-10, 1963) was compared with the UVAM leakage method in 275 tests; the two tests were found to be in good agreement for the strains tested.
本文描述了一种基于分光光度计检测细菌素敏感培养上清液中紫外线吸收物质（UVAM）泄漏的快速且可重复的细菌素分型方法。要获得可重复的结果且均值标准误差波动不显著，前提条件是：一组在特定条件下产生且强度已确定的标准化细菌素。这些细菌素必须与悬浮的未知细菌培养物以给定比例相互作用，以实现最佳的相互作用复数。在275次测试中，将吉利斯（《卫生学杂志》62:1 - 10, 1963）的“刮擦划线”技术进行的绿脓菌素和大肠杆菌素分型与UVAM泄漏法进行了比较；发现这两种测试对所测试的菌株结果吻合良好。
