Bauernfeind A, Petermüller C, Burrows J R
Zentralbl Bakteriol Orig A. 1978 Apr;240(2):271-8.
The Procedure for pyocin typing of Pseudomonas aeruginosa isolations as proposed by GILLIES and GOVAN has been modified in the following aspects: strains are arranged in a symmetrical way, with a central producer zone and radial indicator streaks, optimal size of inocula for both producer (300 x 10(6)/ml) and indicators (50 X 10(6)/ml) was determined, unsupplemented Tryptic Soy Agar is used giving identical results to those when TSA is supplemented with 5% horse blood. The typing results obtained with this simplified and standardized procedure were identical with those from the cross streak method.
菌株以对称方式排列，有一个中央产生菌区和放射状指示菌划线，确定了产生菌（300×10⁶/ml）和指示菌（50×10⁶/ml）的最佳接种量，使用未添加补充剂的胰蛋白胨大豆琼脂，其结果与添加5%马血的胰蛋白胨大豆琼脂相同。用这种简化和标准化方法获得的分型结果与交叉划线法的结果相同。
