Karsten U, Rudolph M
Arch Geschwulstforsch. 1985;55(5):305-10.
In experiments aimed at generating monoclonal antibodies against tumour associated antigens, mice are usually immunized with cancer cells (or membrane fractions prepared from it) obtained from surgery or cultured in vitro. In both situations there is the danger of introducing mycoplasmas into the hybridoma cultures even if the myeloma cell line and the mice used for immunization or feeder cell preparation are not infected. Mycoplasmas kill hybridoma cells during HAT selection because they excessively degrade thymidine. The use of azaserine instead of aminopterin in hybrid selection circumvents the necessity for thymidine and therefore may allow survival and growth of hybridomas in the presence of mycoplasmas.
在旨在生成针对肿瘤相关抗原的单克隆抗体的实验中，通常用从手术获取或体外培养的癌细胞（或由其制备的膜组分）对小鼠进行免疫。在这两种情况下，即使骨髓瘤细胞系以及用于免疫或制备饲养细胞的小鼠未被感染，也存在将支原体引入杂交瘤培养物的风险。支原体在HAT选择过程中会杀死杂交瘤细胞，因为它们会过度降解胸腺嘧啶核苷。在杂交瘤选择中使用重氮丝氨酸而非氨基蝶呤可避免对胸腺嘧啶核苷的需求，因此在存在支原体的情况下可能使杂交瘤存活并生长。
