用于检测感染细胞的支原体的单克隆抗体的研制与应用

Development and application of monoclonal antibodies for the detection of cell infecting mycoplasmas.

作者信息

Douma R, Dular R, Brodeur B R, Kasatiya S S

机构信息

National Laboratory for Immunology, Laboratory Centre for Disease Control, Health and Welfare Canada, Ottawa, Ontario.

出版信息

J Immunol Methods. 1989 Nov 30;124(2):197-203. doi: 10.1016/0022-1759(89)90353-0.

DOI:10.1016/0022-1759(89)90353-0

PMID:2513356

Abstract

Two hybridoma cell lines producing monoclonal antibodies (MAbs) to mycoplasmas were established by fusion of mouse myeloma and spleen cells obtained from mice immunized with whole cell Mycoplasma pneumoniae antigens. The MAbs designated as MYC-4 and MYC-9 bound to a single M. pneumoniae protein band with approximate molecular weights of 150,000. In dot-EIA both MAbs reacted with ten Mycoplasma spp., two serovars of Ureaplasma urealyticum and Acholeplasma laidlawii. The MAbs have great potential to be used as an antibody probe for the rapid screening of cell cultures and hybridomas for mycoplasmal contamination.

摘要

通过将小鼠骨髓瘤细胞与用肺炎支原体全细胞抗原免疫的小鼠脾脏细胞融合，建立了两种产生抗支原体单克隆抗体（MAb）的杂交瘤细胞系。命名为MYC-4和MYC-9的单克隆抗体与一条分子量约为150,000的肺炎支原体单一蛋白条带结合。在斑点酶免疫测定中，两种单克隆抗体均与十种支原体、解脲脲原体的两个血清型和莱氏无胆甾原体发生反应。这些单克隆抗体具有很大的潜力，可作为抗体探针用于快速筛选细胞培养物和杂交瘤中的支原体污染。