Kataoka K, Takeoka Y, Hirano S
Arch Histol Jpn. 1985 Jul;48(3):327-39. doi: 10.1679/aohc.48.327.
The gastric mucosa of adult mice was observed by electron microscopy, and the following findings were obtained. Surface mucous cells mostly undergo degeneration in situ before extrusion from the mucosal surface. Degenerating cells exhibit low electron density of the cytoplasmic matrix and interchromatin region of the nucleus. Some vacuoles can be seen in the cytoplasm. The rough endoplasmic reticulum and Golgi complex retain their normal configurations. Mitochondria are condensed. Lysosomes increase in number, and acid phosphatase activity is restricted within them. Massive exocytotic release of mucus is seen at the cell apex. The basolateral plasmalemma seems intact until the latest stage of extrusion. At the tight and gap junctions, the outer leaflets of apposing plasmalemmas remain fused. On the other hand, microfilaments and tonofilaments are dissociated from the intermediate junctions and desmosomes, respectively, during degeneration. Massive discharge of mucus and well preserved basolateral plasmalemma of the degenerating cell may restrict the back-diffusion of gastric juice into the mucosa to a minimum level during the degeneration and extrusion processes.
通过电子显微镜观察成年小鼠的胃黏膜，获得了以下发现。表面黏液细胞在从黏膜表面挤出之前大多在原位发生退化。退化细胞的细胞质基质和细胞核染色质间区域呈现低电子密度。细胞质中可见一些空泡。粗面内质网和高尔基体保持正常形态。线粒体浓缩。溶酶体数量增加，酸性磷酸酶活性局限于其中。在细胞顶端可见大量黏液的胞吐释放。基底外侧质膜在挤出的最后阶段之前似乎保持完整。在紧密连接和缝隙连接处，相邻质膜的外小叶保持融合。另一方面，在退化过程中，微丝和张力丝分别从中间连接和桥粒解离。退化细胞大量分泌黏液以及基底外侧质膜保存完好，这可能在退化和挤出过程中将胃液向黏膜的反向扩散限制到最低水平。
