咀嚼润滑作用。碳水化合物在唾液糖蛋白 - 白蛋白复合物润滑特性中的作用。
Masticatory lubrication. The role of carbohydrate in the lubricating property of a salivary glycoprotein-albumin complex.
作者信息
Hatton M N, Loomis R E, Levine M J, Tabak L A
出版信息
Biochem J. 1985 Sep 15;230(3):817-20. doi: 10.1042/bj2300817.
Abstract
We report for the first time a masticatory-lubrication assay system to assess the lubricating properties of salivary constituents. The lubricating ability of the proline-rich glycoprotein (PRG) of parotid saliva was enhanced by human serum albumin. The interactive effect of albumin was abolished by chemically deglycosylating the glycoprotein. Fluorescence spectroscopy with a hydrophobic probe verified the existence of a PRG-albumin complex and demonstrated that deglycosylation of the PRG altered the nature of its interaction with albumin.
摘要
我们首次报道了一种咀嚼润滑测定系统，用于评估唾液成分的润滑特性。腮腺唾液中富含脯氨酸的糖蛋白（PRG）的润滑能力因人类血清白蛋白而增强。通过对糖蛋白进行化学去糖基化，白蛋白的相互作用效应被消除。使用疏水探针的荧光光谱法证实了PRG-白蛋白复合物的存在，并表明PRG的去糖基化改变了其与白蛋白相互作用的性质。
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