Vinola Selvanathan M J, Mahalaxmi Sekar
Department of Conservative Dentistry and Endodontics, SRM Dental College, Bharathi Salai, Ramapuram, Chennai, 600089, India.
SRM Institute of Science and Technology, Chennai, India.
Sci Rep. 2025 Jul 8;15(1):24413. doi: 10.1038/s41598-025-08761-1.
AH Plus, an epoxy resin-based sealer extensively employed in endodontics, is renowned for its excellent dimensional stability and effective adhesion to dentin. Nevertheless, extensive clinical research has documented its propensity to induce inflammation, especially when freshly mixed. Phytochemicals such as petasin, pachymic acid, curcumin, and shilajit are known for their anti-inflammatory and analgesic properties. This study aimed to investigate the influence of these phytochemicals on cytotoxicity and anti-inflammatory potential of AH Plus. Experimental groups included AH Plus-resin sealer (AHR) with and without 10% petasin (AHP), 0.75% pachymic acid (AHA), 0.5% curcumin (AHC) and 6% shilajit (AHS) at ratios of 5:1 and 10:1 respectively. Stem cells of apical papilla (SCAP) were exposed to the experimental sealers and assessed for cytotoxicity (MTT assay) and cytokine expression levels of IL-6, IL-8 and TNF-α (ELISA) at different time intervals (0 h, 24 h and 48 h). Experiments were performed in triplicate and analyzed using one-way ANOVA (p < 0.05). All experimental groups demonstrated varying levels of decreased cytotoxicity and cytokine expression compared to the control group; specifically in 5:1 ratio. Thus, it can be concluded that addition of petasin, pachymic acid, curcumin, and shilajit with AH Plus sealer in 5:1 ratio resulted in decreased cytotoxicity and enhanced anti-inflammatory properties.
AH Plus是一种广泛应用于牙髓病学的环氧树脂基封闭剂，以其出色的尺寸稳定性和对牙本质的有效粘附性而闻名。然而，大量临床研究记录了它诱发炎症的倾向，尤其是在刚混合时。诸如蜂斗菜素、茯苓酸、姜黄素和希拉季特等植物化学物质以其抗炎和镇痛特性而闻名。本研究旨在探讨这些植物化学物质对AH Plus的细胞毒性和抗炎潜力的影响。实验组包括分别以5:1和10:1的比例添加和不添加10%蜂斗菜素（AHP）、0.75%茯苓酸（AHA）、0.5%姜黄素（AHC）和6%希拉季特（AHS）的AH Plus树脂封闭剂（AHR）。将根尖乳头干细胞（SCAP）暴露于实验性封闭剂中，并在不同时间间隔（0小时、24小时和48小时）评估细胞毒性（MTT试验）以及白细胞介素-6、白细胞介素-8和肿瘤坏死因子-α的细胞因子表达水平（酶联免疫吸附测定）。实验重复进行三次，并使用单因素方差分析进行分析（p < 0.05）。与对照组相比，所有实验组均表现出不同程度的细胞毒性降低和细胞因子表达减少；特别是在5:1的比例下。因此，可以得出结论，以5:1的比例将蜂斗菜素、茯苓酸、姜黄素和希拉季特与AH Plus封闭剂混合可降低细胞毒性并增强抗炎特性。
