Vinola Selvanathan M J, Karthikeyan Kittappa, Sharma Apoorva, Sudheshna Sai, Sekar Mahalaxmi
Department of Conservative Dentistry and Endodontics, SRM Dental College, SRM Institute of Science and Technology, Chennai, Tamil Nadu, India.
J Conserv Dent. 2021 Nov-Dec;24(6):539-543. doi: 10.4103/jcd.jcd_433_21. Epub 2022 Apr 1.
The eugenol from zinc oxide eugenol (ZnOE) sealer tends to diffuse to the periapical region resulting in inflammation. Several modifications of ZnOE sealer have been formulated to minimize the inflammatory potential of the ZnOE sealer. contains petasin which possesses anti-inflammatory property used in treatment of migraine and allergic rhinitis.
The aim of the study was to evaluate the anti-inflammatory property of petasin-incorporated ZnOE sealer on zebrafish.
The study has been reviewed and approved by the Institutional Review Board (SRMU/MandHS/SRMDC/2018/S/025) and by the in-house Institutional Animal Ethics Committee (IAEC) of Pentagrit Research Lab and conducted with compliance to ICH harmonization and principles for animal housing and handling (IAEC Study No: 215/Go06/IAEC). The samples were implanted in the caudal portion of the zebrafish. The samples ( = 50) were divided into five groups ( = 10) - Group 1: incision only (negative control), Group 2: zinc oxide (ZnO) (positive control), Group 3: ZnO + eugenol and petasin in ratio of 10:1, Group 4: ZnO + eugenol and petasin in ratio of 5:1, and Group 5: ZnO + eugenol and petasin in ratio of 1:1. The experimental groups were further subdivided into two subgroups based on time intervals at 24 h and 48 h. The tissue samples were assessed using smear pathology test, and the percentage of inflammation was evaluated. The data were statistically analyzed using SPSS software version 22.0 with a significance level fixed as 5% (α = 0.05).
The presence of inflammatory cells and congestion of arterioles were taken as the criteria to assess inflammatory action. It was maximum in ZnOE sealer followed by ZnOE sealer modified with the addition of petasin to eugenol in the ratio of 10:1, 5:1, and 1:1, respectively.
The addition of petasin extract to ZnOE reduces the inflammation potential of ZnOE sealers. ZnOE sealer containing eugenol and petasin in the ratio of 1:1 showed a maximal reduction in inflammation.
氧化锌丁香酚（ZnOE）封闭剂中的丁香酚易于扩散至根尖周区域，从而引发炎症。人们已对ZnOE封闭剂进行了多种改良，以尽量降低其引发炎症的可能性。[此处文本似乎不完整，“contains petasin which possesses anti-inflammatory property used in treatment of migraine and allergic rhinitis.”这句话前面应该还有相关内容说明是什么含有岩白菜素]含有岩白菜素，该物质具有抗炎特性，可用于治疗偏头痛和过敏性鼻炎。
本研究旨在评估添加岩白菜素的ZnOE封闭剂对斑马鱼的抗炎特性。
本研究已通过机构审查委员会（SRMU/MandHS/SRMDC/2018/S/025）以及Pentagrit研究实验室的内部机构动物伦理委员会（IAEC）的审查与批准，并按照国际人用药品注册技术协调会（ICH）的协调统一要求以及动物饲养与处理原则进行（IAEC研究编号：215/Go06/IAEC）。将样本植入斑马鱼的尾部。样本（n = 50）分为五组（每组n = 10）——第1组：仅做切口（阴性对照），第2组：氧化锌（ZnO）（阳性对照），第3组：ZnO + 丁香酚与岩白菜素按10:1的比例混合，第4组：ZnO + 丁香酚与岩白菜素按5:1的比例混合，第5组：ZnO + 丁香酚与岩白菜素按1:1的比例混合。根据24小时和48小时的时间间隔，将实验组进一步细分为两个亚组。使用涂片病理学检测对组织样本进行评估，并评估炎症百分比。使用SPSS 22.0软件对数据进行统计学分析，显著性水平设定为5%（α = 0.05）。
以炎性细胞的存在和小动脉充血作为评估炎症作用的标准。在ZnOE封闭剂中炎症最为严重，其次是分别添加了比例为10:1、5:1和1:1的岩白菜素的改良ZnOE封闭剂。
向ZnOE中添加岩白菜素提取物可降低ZnOE封闭剂的炎症可能性。丁香酚与岩白菜素比例为1:1的ZnOE封闭剂在炎症方面的降低程度最大。
