Margono Anggraini, Pratiwi Redho Sara, Nofarina Anggita Dini, Npa Dewa Ayu, Maharti Ike Dwi, Rosseti Romilda
Department of Conservative Dentistry, Faculty of Dentistry, Universitas Indonesia, Jakarta, Indonesia.
Eur J Dent. 2025 Jul;19(3):777-783. doi: 10.1055/s-0045-1802566. Epub 2025 May 1.
This article evaluates the biocompatibility and bioactivity of a novel calcium silicate-based sealer by assessing its impact on the viability, mineral deposition, wound closure, and migration activity of human dental pulp stem cells (hDPSCs).
AH Plus and AH Plus Bioceramic were pulverized and sterilized according to International Organization for Standardization 10993-5:2009. The hDPSCs were stored raw materials, reaching 80% confluence after passing stem cell marker tests (CD90 98%, CD105 99.7%, CD73 94%, and LinNeg 0.5%) and were at passage 5 to 6 after serum starvation for 24 hours. The study consisted of four groups: AH Plus at concentrations of 1:1 and 1:4, and AH Plus Bioceramic at concentrations of 1:1 and 1:4. Viability was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay by measuring optical density values, while mineral deposition was evaluated through Alizarin red staining and analyzed with ImageJ software. Migration activity was measured by calculating migration speed and wound closure percentage using a scratch assay at 24 and 72 hours, with results analyzed by ImageJ.
Viability, migration, and wound closure results were analyzed using one-way analysis of variance. Mineral deposition was analyzed descriptively.
There were significant differences in the viability and mineral deposition of hDPSCs between calcium silicate-based sealers and epoxy resin-based sealers. Calcium silicate-based sealers showed higher viability and better mineral deposition. The migration speed rate of hDPSCs at 24 hours and wound closure at 24 and 72 hours were significantly greater with the novel calcium silicate-based sealer compared with the epoxy resin-based sealer.
This study suggests that calcium silicate-based sealers offer advantages over traditional epoxy resin-based sealers, demonstrating superior biocompatibility and bioactivity. These properties may lead to improved clinical outcomes, such as faster healing and fewer posttreatment complications. Further research is needed to explore the full potential of these materials in endodontics.
本文通过评估一种新型硅酸钙基封闭剂对人牙髓干细胞（hDPSC）的活力、矿物质沉积、伤口闭合及迁移活性的影响，来评价其生物相容性和生物活性。
按照国际标准化组织10993 - 5:2009对AH Plus和AH Plus生物陶瓷进行粉碎和灭菌。hDPSC作为储存原料，经干细胞标志物检测（CD90 98%、CD105 99.7%、CD73 94%、LinNeg 0.5%）后达到80%汇合度，血清饥饿24小时后处于第5至6代。该研究分为四组：浓度为1:1和1:4的AH Plus，以及浓度为1:1和1:4的AH Plus生物陶瓷。通过测量光密度值，使用3 -（4,5 - 二甲基噻唑 - 2 - 基）- 2,5 - 二苯基四氮唑溴盐（MTT）法评估活力，而通过茜素红染色评估矿物质沉积并用ImageJ软件进行分析。迁移活性通过在24小时和72小时使用划痕试验计算迁移速度和伤口闭合百分比来测量，结果用ImageJ分析。
活力、迁移和伤口闭合结果采用单因素方差分析。矿物质沉积进行描述性分析。
硅酸钙基封闭剂和环氧树脂基封闭剂在hDPSC的活力和矿物质沉积方面存在显著差异。硅酸钙基封闭剂显示出更高的活力和更好的矿物质沉积。与环氧树脂基封闭剂相比，新型硅酸钙基封闭剂在24小时时hDPSC的迁移速度以及24小时和72小时时的伤口闭合情况均显著更好。
本研究表明，硅酸钙基封闭剂比传统的环氧树脂基封闭剂具有优势，显示出卓越的生物相容性和生物活性。这些特性可能导致临床效果改善，如愈合更快和治疗后并发症更少。需要进一步研究以探索这些材料在牙髓病学中的全部潜力。
