在存在或不存在产物抑制的情况下，对高浓度米氏酶的进程曲线进行分析。
Analysis of progress curves for a highly concentrated Michaelian enzyme in the presence or absence of product inhibition.
作者信息
Kellershohn N, Laurent M
出版信息
Biochem J. 1985 Oct 1;231(1):65-74. doi: 10.1042/bj2310065.
Abstract
Methods are given for analysing the time course of an enzyme-catalysed reaction when the concentration of the enzyme itself is high, a situation which is often found in vivo. (1) The integrated form of the kinetic equation for a concentrated Michaelian enzyme in absence of product inhibition is given. Parameters are shown to be calculated easily using non-linear fitting procedures. (2) A general algorithm to analyse progress-curve data in more complex cases (i.e. when the analytical form of the integrated rate equation is not known or is exceedingly complex) is proposed. This algorithm may be used for any enzyme mechanism for which the differential form of the kinetic equation may be written analytically. We show that the method allows differentiation between the main types of product inhibition which may occur in the case of a highly concentrated Michaelian enzyme.
摘要
文中给出了在酶自身浓度较高（这在体内常常出现）的情况下分析酶催化反应时间进程的方法。(1)给出了不存在产物抑制时浓米氏酶动力学方程的积分形式。结果表明，使用非线性拟合程序可以轻松计算参数。(2)提出了一种用于分析更复杂情况下进程曲线数据的通用算法（即当积分速率方程的解析形式未知或极其复杂时）。该算法可用于任何能以解析形式写出动力学方程微分形式的酶机制。我们表明，该方法能够区分在高浓度米氏酶情况下可能出现的主要产物抑制类型。
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