Amanuma K, Okada J, Imanaka T, Ohkuma S, Takano T
Biochem Int. 1985 Sep;11(3):349-55.
The intracellular localization of acid lipase was examined by several subcellular fractionation techniques. Upon differential centrifugation, acid lipase was detected mainly in the lysosomal fraction. When linear sucrose density gradient centrifugation was used, the mean density of acid lipase was similar to that of lysosomal marker enzymes. After Triton-treatment of rats, however, acid lipase was recovered at a density of 1.10-1.16, which is more than that of tritosomes, when tissues were homogenized with a Potter-Elvehjem homogenizer. But by homogenizing the liver from these rats by pressing it through a stainless steel net, acid lipase was mainly recovered at the same density as that of tritosomes. These results indicate that acid lipase is localized mainly in lysosomes in rat liver.
通过几种亚细胞分级分离技术研究了酸性脂肪酶的细胞内定位。差速离心后，酸性脂肪酶主要在溶酶体组分中检测到。当使用线性蔗糖密度梯度离心时，酸性脂肪酶的平均密度与溶酶体标记酶的相似。然而，在用波特-埃尔维耶姆匀浆器对大鼠进行曲拉通处理后，当用不锈钢网挤压匀浆这些大鼠的肝脏时，酸性脂肪酶在密度为1.10 - 1.16处回收，这一密度高于三体溶酶体；但此时酸性脂肪酶主要在与三体溶酶体相同的密度处回收。这些结果表明酸性脂肪酶主要定位于大鼠肝脏的溶酶体中。
