Fujita K, Fujita H M
Int J Clin Pharmacol Ther Toxicol. 1985 Jun;23(6):288-90.
Rat kidney was homogenized and centrifuged by continuous sucrose gradient density. Aminoglycosides and beta-lactam antibiotics were localized in the lysosomes, whereas the localization of tetracyclines was in the mitochondria. This study demonstrated a technique for the localization of antibiotics in the subcellular fractions without the use of a radioactive tracer.
将大鼠肾脏进行匀浆，并通过连续蔗糖梯度密度离心法进行离心。氨基糖苷类和β-内酰胺类抗生素定位于溶酶体中，而四环素类的定位在线粒体中。本研究展示了一种在不使用放射性示踪剂的情况下将抗生素定位于亚细胞组分的技术。
